For this validation, we compared our current freezing medias to Cryostor10 in order
to determine the efficacy of this commercially available product for QC vial cryopreservation.
We collected 10 allogeneic Hematopoietic Progenitor Cell patient products, 5 HPC Apheresis
and 5 HPC Marrow. Our current method consists of freezing four nunc vials each with
15 × 106 cells for HPC Apheresis and 30 × 106 cells for HPC Marrow using 2 mL of premade freezing solution (RPMI, FBS, Hepes Buffer,
and Penicillin) in combination with 2 mL freezing media (RPMI, DMSO, EDTA and Sodium
Bicarbonate). In this validation 2 additional vials of cells were cryopreserved in
2 mL of CryoStor10 post processing alongside control vials which were prepared using
our current approved freezing method. The vials were stored overnight in a −80 freezer
and then transferred to LN2 storage.
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