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Cryopreservation
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Our library of independent research demonstrates the effectiveness of BioLife Solutions’ products.
Over 600 independent research projects show how BioLife Solutions products improve sample viability.
Over 600 Articles, Abstracts and Posters Citing the Use of CryoStor and HypoThermosol.
Hundreds of articles have been written about the use of our products, CryoStor and HypoThermosol. Learn more about our products’ use in the cell and gene therapy industry, or ask one of our scientists a question directly.
Presentation
Journal Articles
Abstracts
Posters
Domingues, S., Darle, A., Masson, Y., Saidani, M., Lagoutte, E., Bejanariu, A., Coutier, J., Ayata, R. E., Bouschbacher, M., Peschanski, M., Lemaitre, G. and Baldeschi, C. Clinical Grade Human Pluripotent Stem Cell-Derived Engineered Skin Substitutes Promote Keratinocytes Wound Closure In Vitro. Cells. 2022; 11(7), 1151. doi: 10.3390/cells11071151
Iwata, Y., Narushima, Y., Harada, A. and Mishima, M. Priming treatment with T-cell redirecting bispecific antibody ERY974 reduced cytokine induction without losing cytotoxic activity in vitro by changing the chromatin state in T cells. Toxicology And Applied Pharmacology. 2022; 441, 115986. doi: 10.1016/j.taap.2022.115986
Thongsin, N. and Wattanapanitch, M. A three-dimensional immune-oncology model for studying in vitro primary human NK cell cytotoxic activity. PLOS ONE. 2022; 17(3), e0264366. doi: 10.1371/journal.pone.0264366
Aisha Naeem, Nikita Gupta, Natasha Arzoo, Usra Naeem, Muhammad Jawad Khan, Muhammad Umer Choudhry, Wanxing Cui and Chris Albanese. A Survey and Critical Evaluation of Isolation, Culture, and Cryopreservation Methods of Human Amniotic Epithelial Cells. Cell Cycle. 2022. doi: 10.1080/15384101.2021.2020015
Olivarria, G. M., Cheng, Y., Furman, S., Pachow, C., Hohsfield, L. A., Smith-Geater, C., Miramontes, R., Wu, J., Burns, M. S., Tsourmas, K. I., Stocksdale, J., Manlapaz, C., Yong, W. H., Teijaro, J., Edwards, R., Green, K. N., Thompson, L. M. and Lane, T. E. Microglia Do Not Restrict SARS-CoV-2 Replication following Infection of the Central Nervous System of K18-Human ACE2 Transgenic Mice. Journal Of Virology. 2022; 96(4). doi: 10.1128/jvi.01969-21
DeVeaux, S. A., Ogle, M. E., Vyshnya, S., Chiappa, N. F., Leitmann, B., Rudy, R., Day, A., Mortensen, L. J., Kurtzberg, J., Roy, K. and Botchwey, E. A. Characterizing human mesenchymal stromal cells’ immune-modulatory potency using targeted lipidomic profiling of sphingolipids. Cytotherapy. 2022. doi: 10.1016/j.jcyt.2021.12.009
Capelli, C., Frigerio, S., Lisini, D., Nava, S., Gaipa, G., Belotti, D., Cabiati, B., Budelli, S., Lazzari, L., Bagnarino, J., Tanzi, M., Comoli, P., Perico, N., Introna, M. and Golay, J. A comprehensive report of long-term stability data for a range ATMPs: A need to develop guidelines for safe and harmonized stability studies. Cytotherapy. 2022. doi: 10.1016/j.jcyt.2021.12.004
Vogelzang, N. J., Beer, T. M., Gerritsen, W., Oudard, S., Wiechno, P., Kukielka-Budny, B., Samal, V., Hajek, J., Feyerabend, S., Khoo, V., Stenzl, A., Csöszi, T., Filipovic, Z., Goncalves, F., Prokhorov, A., Cheung, E., Hussain, A., Sousa, N., Bahl, A., Hussain, S., Fricke, H., Kadlecova, P., Scheiner, T., Korolkiewicz, R. P., Bartunkova, J., Spisek, R., Stadler, W., Berg, A. S., Kurth, K., Higano, C. S., Aapro, M., Krainer, M., Hruby, S., Meran, J., Polyakov, S., Machiels, J., Roumeguere, T., Ackaert, K., Lumen, N., Gil, T., Minchev, V., Tomova, A., Dimitrov, B., Koleva, M., Juretic, A., Fröbe, A., Vojnovic, Z., Drabek, M., Jarolim, L., Buchler, T., Kindlova, E., Schraml, J., Zemanova, M., Prausova, J., Melichar, B., Chodacka, M., Jansa, J., Daugaard, G., Delonchamps, N., Duclos, B., Culine, S., Deplanque, G., Le Moulec, S., Hammerer, P., Rodemer, G., Ritter, M., Merseburger, A., Grimm, M., Damjanoski, I., Wirth, M., Burmester, M., Miller, K., Herden, J., Keck, B., Wuelfing, C., Winter, A., Boegemann, M., von Schmeling, I. K., Fornara, P., Jaeger, E., Bodoky, G., Pápai, Z., Böszörményi-Nagy, G., Vanella, P., SotoParra, H., Passalacqua, R., Ferrau, F., Maio, M., Fratino, L., Cortesi, E., Purkalne, G., Asadauskiene, J., Janciauskiene, R., Tulyte, S., Cesas, A., Polee, M., Haberkorn, B., van de Eertwegh, F., van den Berg, P., Beeker, A., Nieboer, P., Zdrojowy, R., Staroslawska, E., Fijuth, J., Sikora-Kupis, B., Karaszewska, B., Fernandes, I., Sousa, G., Rodrigues, T., Dzamic, Z., Babovic, N., Cvetkovic, B., Sokol, R., Mikuláš, J., Gajdos, M., Brezovsky, M., Mincik, I., Breza, J., Arranz, J. A., Calvo, V., Rubio, G., Chapado, M. S., Boreu, P. G., Montesa, A., Olmos, D., Mellado, B., Castellano, D., Puente, J., Karlsson, E. T., Ahlgren, J., Pandha, H., Mazhar, D., Vilarino-Varela, M., Elliott, T., Pedley, I., Zarkar, A., Law, A., Slater, D., Karlin, G., Bilusic, M., Redfern, C., Gaur, R., McCroskey, R., Clarkson, D., Agrawal, M., Shtivelband, M., Nordquist, L., Karim, N., Hauke, R., Flaig, T., Jhangiani, H., Singal, R., Choi, B., Reyes, E., Corman, J., Hwang, C., Appleman, L., McClay, E., Fleming, M., Gunuganti, V., Cheung, E., Gartrell, B., Sartor, A., Williamson, S., Gandhi, J., Schnadig, I., Burke, J., Bloom, S., Shore, N., Mayer, T., Oh, W., Bryce, A., Belkoff, L., Vaishampayan, U., Agarwala, S., Kucuk, O., Agrawal, A., Walsh, W., Poiesz, B., Harshman, L., Dawson, N. and Sharma, S. Efficacy and Safety of Autologous Dendritic Cell–Based Immunotherapy, Docetaxel, and Prednisone vs Placebo in Patients With Metastatic Castration-Resistant Prostate Cancer – The VIABLE Phase 3 Randomized Clinical Trial. JAMA Oncology. 2022. doi: 10.1001/jamaoncol.2021.7298
Fear, V. S., Forbes, C. A., Anderson, D., Rauschert, S., Syn, G., Shaw, N., Jamieson, S., Ward, M., Baynam, G. and Lassmann, T. CRISPR single base editing, neuronal disease modelling and functional genomics for genetic variant analysis: pipeline validation using Kleefstra syndrome EHMT1 haploinsufficiency. Stem Cell Research & Therapy. 2022; 13(1). doi: 10.1186/s13287-022-02740-3
Bifani, A. M., Tan, H. C., Choy, M. M., and Ooi, E. E. Cell Strain-Derived Induced Pluripotent Stem Cells as an Isogenic Approach To Investigate Age-Related Host Response to Flaviviral Infection. Journal of Virology. 2022; 96(3). doi: 10.1128/jvi.01737-21
Kakon, N., Luizaga, H., Pham, C., Bezabih, R., Elnour, H., Procter, J., Ping, J., Highfill, S., Stroncek, D. F., Panc, S. R. Comparable Postthaw CD34+ and CD3+ Cell Characteristics With Commercial and In-House Cryoprotectants: Commercial Favored for Ease of Use. Transfusion. 2019; 59(S3), 106A-107A. doi:10.1111/trf.15462
Lee, Tsung (John) Dao. A Comprehensive Review of Umbilical Cord Cryopreservation: Umbilical Cord Tissue Banking Cryopreserved Technology and Standard Operating Procedure. Transfusion Vol. 58, 2018 Supplement. Pages 4A-5Av.
Clark, E., Stasko, K., and Paciulan, B. A COMPARISON STUDY OF CURRENT FREEZING MEDIA VERSUS CRYOSTOR10 FOR CRYOPRESERVATION OF QC VIALS. Cytotherapy. May 2018; 20(5), S62. doi.org/10.1016/j.jcyt.2018.02.173
Pasha, Elmoazzen, Pineault. Development and testing of a stepwise thaw and dilute protocol for cryopreserved umbilical cord blood units.Transfusion. 2017 Jul;57(7):1744-1754. doi: 10.1111/trf.14136
Biunno I, Ntai A. Banking of Human Pluripotent Stem Cells. Biopreservation and Biobanking Volume 15, Number 3, 2017. DOI:10.1089=bio.2017.29021.abstracts
Fink J, Abazari A, Hawkins B, Albert M, O’Donnell K, Mathew A. Best Practices vs. Traditional Practices of Procedures and Technology for Preserving Viability and Functionality of T-Cells at Both -80 C and -190 C. Biopreservation and Biobanking Volume 15, Number 3, 2017. DOI:10.1089=bio.2017.29021.abstracts
Abazari A. Biopreservation considerations in the evolving field of cellular therapies. Cryobiology Volume 73, Issue 3, December 2016, Page 399. https://doi.org/10.1016/j.cryobiol.2016.09.007
de Vries M, Broeren MGA, Bennink MB, et al. A10.08 Detailed analysis of the effect of cryopreservation on the viability and cytokine release of human synovial tissue. Annals of the Rheumatic Diseases 2016; 75:A75-A76. doi:10.1136/annrheumdis-2016-209124.180.
Gramlich OW, Zhu W, Burand A, Ankrum JA, et al. Transplantation of cryopreserved MSC rescues retinal ganglion cells following I/R injury. ARVO 2016 Annual Meeting Abstracts (2016).
Fearnot ER, Fazekasova H, Thirkell S, Lowe K, Bushell A, Lombardi G. C-25: Evaluation of closed system medical device for low volume storage for clinical studies involving regulatory T cells. Cryobiology (2014). Volume 69, Issue 3, December 2014. Pages 508. doi:10.1016/j.cryobiol.2014.09.312
Hypothermic Storage & Shipping Media
Presentation
Journal Articles
Abstracts
Posters
Won, T., Wood, M. K., Hughes, D. M., Talor, M. V., Ma, Z., Schneider, J., Skinner, J. T., Asady, B., Goerlich, E., Halushka, M. K., Hays, A. G., Kim, D., Parikh, C. R., Rosenberg, A. Z., Coppens, I., Johns, R. A., Gilotra, N. A., Hooper, J. E., Pekosz, A. and Čiháková, D. Endothelial thrombomodulin downregulation caused by hypoxia contributes to severe infiltration and coagulopathy in COVID-19 patient lungs. Ebiomedicine. 2022; 75, 103812. doi: 10.1016/j.ebiom.2022.103812
Erol, O. D., Pervin, B., Seker, M. E. and Aerts-Kaya, F. Effects of storage media, supplements and cryopreservation methods on quality of stem cells. World Journal Of Stem Cells. 2021; 13(9), 1197-1214. doi: 10.4252/wjsc.v13.i9.1197
Ciervo, Y., Gatto, N., Allen, C., Grierson, A., Ferraiuolo, L., Mead, R. J. and Shaw, P. J. Adipose-derived stem cells protect motor neurons and reduce glial activation in both in vitro and in vivo models of ALS. Molecular Therapy – Methods & Clinical Development. 2021 Mar 27; 21:413-433. doi: 10.1016/j.omtm.2021.03.017
Ścieżyńska, A., Soszyńska, M., Szpak, P., Krześniak, N., Malejczyk, J. and Kalaszczyńska, I. Influence of Hypothermic Storage Fluids on Mesenchymal Stem Cell Stability: A Comprehensive Review and Personal Experience. Cells. 2021; 10(5), 1043. doi: 10.3390/cells10051043
Nam, Y., Yoon, D., Hong, J., Kim, M. S., Lee, T. Y., Kim, K. S., Lee, H., Suk, K. and Kim, S. R. Therapeutic Effects of Human Mesenchymal Stem Cells in a Mouse Model of Cerebellar Ataxia with Neuroinflammation. Journal Of Clinical Medicine. 2021; 9(11), 3654. doi: 10.3390/jcm9113654
Todd CJ Berard, Aby J Mathew. Biopreservation and cold chain biologistics risk points in the cell and gene therapy workflow. Cell & Gene Therapy Insights 2020; 6(08), 1363–1380. doi: 10.18609/cgti.2020.150
Singh, S., Chakravarty, T., Chen, P., Akhmerov, A., Falk, J., Friedman, O., Zaman, T., Ebinger, J. E., Gheorghiu, M., Marbán, L., Marbán, E. and Makkar, R. R. Allogeneic cardiosphere-derived cells (CAP-1002) in critically ill COVID-19 patients: compassionate-use case series. Basic Research In Cardiology. 2020; 115(4). doi: 10.1007/s00395-020-0795-1
Mund, S. J. K., Kawamura, E., Awang-Junaidi, A. H., Campbell, J., Wobeser, B., MacPhee, D. J., Honaramooz, A. and Barber, S. Homing and Engraftment of Intravenously Administered Equine Cord Blood-Derived Multipotent Mesenchymal Stromal Cells to Surgically Created Cutaneous Wound in Horses: A Pilot Project. Cells. 2020; 9(5), 1162. doi: 10.3390/cells9051162
Menon, R., Otto, E. A., Hoover, P. J., Eddy, S., Mariani, L. H., Godfrey, B., Berthier, C. C., Eichinger, F., Subramanian, L., Harder, J. L., Ju, W., Nair, V., Larkina, M., Naik, A. S., Luo, J., jain, S., Sealfon, R., Troyanskaya, O. G., Hacohen, N., Hodgin, J. B. and Kretzler, M. Single cell transcriptomics identifies focal segmental glomerulosclerosis remission endothelial biomarker. JCI Insight. 2020; 5(6):e133267. doi:10.1172/jci.insight.133267
Madissoon, E., Wilbrey-Clark, A., Miragaia, R. J., Saeb-Parsy, K., Mahbubani, K. T., Georgakopoulos, N., Harding, P., Polanski, K., Huang, N., Nowicki-Osuch, K., Fitzgerald, R. C., Loudon, K. W., Ferdinand, J. R., Clatworthy, M. R., Tsingene, A., van Dongen, S., Dabrowska, M., Patel, M., Stubbington, M. J. T., Teichmann, S. A., Stegle, O. and Meyer, K. B. scRNA-seq assessment of the human lung, spleen, and esophagus tissue stability after cold preservation. Genome Biology. 2020; 21(1). doi: 10.1186/s13059-019-1906-x
Abazari A. Biopreservation considerations in the evolving field of cellular therapies. Cryobiology Volume 73, Issue 3, December 2016, Page 399. https://doi.org/10.1016/j.cryobiol.2016.09.007
Liotta A, Sandersen C, Ceusters J, Girod M, Peeters D, Bolen G. Computed tomography-guided injection of muscle-derived mesenchimal stem cells in the intervertebral disc of dogs affected by natural disc degeneration: clinical safety and intervertebral disc imaging assessment.
Rossetti T, Nichols F, Modo M. Intracerebral Cell Implantation: Preparation and Characterization of Cell Suspensions. Cell Transplant. 2016;25(4):645-664. doi: 10.3727/096368915X690350
Herias M. Investigation of HypoThermosol® as a delivery medium for cardiomyocyte progenitor cells. Cytotherapy 16(4):S38 (2014). DOI: https://dx.doi.org/10.1016/j.jcyt.2014.01.130
Honaramooz, Yang. Isolation of Porcine Testis Cells from Fresh and Preserved Tissues. Biology of Reproduction, Volume 81, Issue Suppl_1, 1 July 2009, Page 688. https://doi.org/10.1093/biolreprod/81.s1.688
Snyder KK, Baust JM, Van Buskirk RG, Baust JG. Improved Hypothermic Storage of Sensitive Primary Cell Systems. Cytotherapy, Vol. 7 Supp. 1, pg. 2, 2005.
Snyder KK, Baust JM, Van Buskirk RG, Baust JG. Assessment of Function and Metabolic Activity of Neonatal Cardiomyocytes Following Hypothermic Storage. Cryobiology, Vol. 47 No. 3, pg. 273, 2003. (P.L. Steponkus Crystal Award Recipient)
Mathew AJ, Baust JG, Van Buskirk RG. Enhanced hypothermic preservation of multiple human cell types. Cryobiology 43:383 (2001).
Mathew AJ, Baust JG, Van Buskirk RG. Enhanced hypothermic preservation of human renal cells. Cryobiology 43:357 (2001).
Mathew, AJ, Baust JM, Hollister WR, Al-Abdullah IH, Ricordi C, Van Buskirk RG, Baust JG. Molecular biological analysis of cell death leads to improvement in the hypothermic solution, HypoThermosol. Cryobiology 41:335-6 (2000).
