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Biopreserved in Home-Brew Media

Biopreserved in HypoThermosol

EXTEND CELL STABILITY

After three days in the cold, do your cells look like those on the left or those stored in HypoThermosol?

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EVIDENCE

Will CryoStor freeze media and HypoThermosol storage media preserve cells, tissues, and organs better than your alternatives?

We have dozens of studies, tests, and reviews that say “Yes, BioLife products outperform commercially available alternatives and ‘home-brew’ biopreservation media.”

If you want to extend cell stability, improve cell viability, and lower the average cost per delivered dose of your cell therapy product (arguably the most important metric once safety and efficacy are proven), read on.

Search Our Evidence

Cryopreservation Freeze Media

Woods EJ, Thirumala S, Badhe-Buchanan SS, Clarke D, Mathew AJ. Off the shelf cellular therapeutics: Factors to consider during cryopreservation and storage of human cells for clinical use. (2016). Cytotherapy, June 2016, 18;(6), p697-711. DOI: http://dx.doi.org/10.1016/j.jcyt.2016.03.295.

Fernandes, P. et al. Canine helper-dependent vectors production: implications of Cre activity and co-infection on adenovirus propagation. Scientific Reports 5, Article number: 9135, 16 March 2015 doi:10.1038/srep09135

Fritsch G, Frank N, Dmytrus J, Frech C, Pichler H, Witt V, Geyeregger R, Scharner D, Trbojevic D, Zipperer E, Printz D, Worel N. Relevance of flow cytometric enumberation of post-thaw leucocytes: influence of temperature during cell staining on viable cell recovery. Vox Sanguinis. (2016) April 1. doi: 10.1111/vox.12398. [Epub ahead of print]

Telezhkin V, Schnell C, Yarova P, Yung S, Cope E, et al. Forced cell cycle exit and modulation of GABAA, CREB, and GSK3β signaling promote functional maturation of induced pluripotent stem cell-derived neurons. American Journal of Physiology – Cell Physiology Published 1 April 2016 Vol. 310 no. 7, C520-C541. DOI: 10.1152/ajpcell.00166.2015

Safinia N, Vaikunthanathan T, Fraser H, Thirkell S, et al. Successful expansion of functional and stable regulatory T Cells for immunotherapy in liver transplantation. Onotarget, Advance Publications (2016) Feb 16;7(7);7563-77. doi: 10.18632/oncotarget.6927

Milani P, Escalante-Chong R, Shelley BC, Patel-Murray NL, Xin X, Adam M, Mandefro B, Sareen D, Svendsen CN, Fraenkel E. Cell freezing protocol optimized for ATAC-Seq on motor neurons derived from human induced pluripotent stem cells. BioRxiv. (2016). Epub on line Jan 15, 2016.

Curbishley SM, Blahava M, Adams DH. Designing a dendritic cell-based therapy for primary liver cancer. MACS&more, Vol 17-1/2016

Fernandes RAB, Koss MJ, Falabella P, Stefanini FR, et al. An Innovative Surgical Technique for Subretinal Transplantation of Human Embryonic Stem Cell-Derived Retinal Pigmented Epithelium in Yucatan Mini Pigs: Preliminary Results. Ophthalmic Surgery, Lasers & Imaging Retina. (2016); Vol. 47, No. 4, pps 342-351. doi: 10.3928/23258160-20160324-07

Fortin JM, Azari H, Zheng T, Darioosh RP, Schmoll, ME, Vedam-Mai V, Deleyrolle LP, Reynolds BA. Transplantation of Defined Populations of Differentiated Human Neural Stem Cell Progeny. Scientific Reports 6, Article number:23579 (2016). doi: 10.1038/srep23579

Singh N, Barnes SJ, Jenwithisuk R, Sattabongkot J, Adams JH. A simple and efficient method for cryopreservation and recovery of viable Plasmodium vivax and P. falciparum sporozoites. Parasitology International (2015) Epub 8 Dec 2015. doi:10.1016/j.parint.2015.12.003

Greene SJ, Epstein SE, Kim RJ, Quyyumi AA, Cole RT, Anderson AS, Wilcox JE, Skopicki HA, Sikora S, Verkh L, Tankovich NI, Gheorghiade M, Butler J. Rationale and design of a randomized controlled trial of allogeneic mesenchymal stem cells in patients with nonischemic cardiomyopathy. Journal of Cardiovascular Medicine, published ahead of print. doi: 10.2459/JCM.0000000000000303

Chen VC, Ye J, Shukla P, Hua G, Chen D, Lin Z, Liu J, Chai J. Development of a scalable suspension culture for cardiac differentiation from human pluripotent stem cells. Stem Cell Research, Volume 15, Issue 2, September 2015, 365–375. doi:10.1016/j.scr.2015.08.002

Hossain DMS, Pal SK, Moreira D, et al. LR9-Targeted STAT3 Silencing Abrogates Immunosuppressive Activity of Myeloid-Derived Suppressor Cells from Prostate Cancer Patients. Author manuscript published online first on May 12, 2015; DOI: 10.1158/1078-0432.CCR-14-3145

Oommen S, Yamada S, Peral SC, Campbell KA, Bruinsma ES, Terzic A, Nelson TJ Human umbilical cord blood-derived mononuclear cells improve murine ventricular function upon intramyocardial delivery in right ventricular chronic pressure overload. Stem Cell Research & Therapy. 2015;6(1):50. doi:10.1186/s13287-015-0044-y

Peral SC, Burkhart HM, Oommen S, Yamada S, et al. Safety and Feasibility for Pediatric Cardiac Regeneration Using Epicardial Delivery of Autologous Umbilical Cord Blood-Derived Mononuclear Cells Established in a Porcine Model System. Stem Cells Tranl Med. 2015 Feb;4(2):195-206. doi: 10.5966/sctm.2014-0195. Epub 2015 Jan 5.

Zhang et al. Sp1 and c-Myc modulate drug resistance of leukemia stem cells by regulating survivin expression through the ERK-MSK MAPK signaling pathway. Molecular Cancer (2015) 14:56 DOI 10.1186/s12943-015-0326-0

Hamot G, Ammerlaan W, Mathay C, Kofanova O, Betsou F. Method validation for automated isolation of viable peripheral blood mononuclear cells. Biopreservation and Biobanking, Vol. 13, No. 3, 2015. Mary Ann Liebert, Inc. DOI: 10.1089/bio.2014.0054

Fernandes P. Canine helper-dependent vectors production: implications of Cre activity and co-infection on adenovirus propagation. Scientific Reports 5, Article number: 9135, 16 March 2015 doi:10.1038/srep09135

AGE Day, Bhangra KS, Murray-Dunning C, Thanabalasundaram L, Grace N, Cameron G, Stevanato L, Sinden J, Phillips JB. Hypothermic and cryogenic preservation of artificial neural tissue made using differentiated CTX human neural stem cells in collagen gels. European Cells and Materials. Vol 29. Suppl. 3, (2015). Pg 44.

Podrazil M, Horvath R, Becht E, et al. Phase I/II clinical trial of dendritic-cell based immunotherapy (DCVAC/PCa) combined with chemotherapy in patients with metastatic, castration-resistant prostate cancer. (2015). Oncotarget, 5, 17891-17904.

Ramos TV, Mathew AJ, Thompson ML, Ehrhardt RO. Standardized cryopreservation of human primary cells. (2014). Curr Protoc Cell Biol. 2014 Sep 2;64:A.3I.1-8. doi:10.1002/0471143030.cba03is64.

Eyrich M, et al. Development and validation of a fully GMP-compliant production process of autologous, tumor-lysate-pulsed dendritic cells. Cytotherapy – July 2014 (Vol. 16, Issue 7, Pages 946-964, DOI: 10.1016/j.jcyt.2014.02.017).

Guzman ML, Yang N, Sharma KK, Balys M, Corbett CA, Jordan CT, Becker MW, Steidl U, Abdel-Wahab O, Levine RL, Marcucci G, Roboz GJ, Hassane DC. Selective Activity of the Histone Deacetylase Inhibitor AR-42 against Leukemia Stem Cells: A Novel Potential Strategy in Acute Myelogenous Leukemia. Mol Cancer Ther 2014;13:1979-1990. Published Online First June 16, 2014.

Zhu WZ, Van Biber B, Laflamme MA. Methods for the Derivation and Use of Cardiomyocytes from Human Pluripotent Stem Cells. Methods Mol Biol. Author manuscript; available in PMC Jun 4, 2014. Published in final edited form as: Methods Mol Biol. 2011; 767: 419–431. doi: 10.1007/978-1-61779-201-4_31

Kofanova Olga A., Davis Kristine, Glazer Barbara, De Souza Yvonne, Kessler Joseph, Betsou Fotini, and for the ISBER Biospecimen Science Working Group. Viable Mononuclear Cell Stability Study for Implementation in a Proficiency Testing Program: Impact of Shipment Conditions. Biopreservation and Biobanking. June 2014, 12(3): 206-216. doi:10.1089/bio.2013.0090.

Kemppinen AK, Baker A, Liao W, et al. Exome sequencing in single cells from the cerebrospinal fluid in multiple sclerosis. Mult Scler 1352458514529613, first published on April 16, 2014 doi:10.1177/1352458514529613

Foussat A, Rietze R, Pottier E, et al. Effective Cryopreservation and Recovery of Human Regulatory T Cells. BioProcess International, Vol. 12, No. S3, March 2014, pp. 34–38.

Callahan KP, Minhajuddin M, Corbett C, Lagadinou ED, Rossi RM, Grose V, Balys MM, Pan L, Jacob S, Frontier A, Grever MR, Lucas DM, Kinghorn AD, Liesveld JL, Becker MW, Jordan CT.. Flavaglines target primitive leukemia cells and enhance anti-leukemia drug activity. Leukemia (28 February 2014) | doi:10.1038/leu.2014.93

Ikebe C, Suzuki K. Mesenchymal Stem Cells for Regenerative Therapy: Optimization of Cell Preparation Protocols. BioMed Research International, vol. 2014, Article ID 951512, 11 pages, 2014. doi:10.1155/2014/951512

Brandl C, Zimmermann SJ, Milenkovic VM, Rosendahl SM, Grassmann F, Milenkovic A, Hehr U, Federlin M, Wetzel CH, Helbiq H, Weber BH.. In-Depth Characterisation of Retinal Pigment Epithelium (RPE) Cells Derived from Human Induced Pluripotent Stem Cells (hiPSC). Neuromol Med (2014) 16:551–564 DOI 10.1007/s12017-014-8308-8

Ramos TV, Wang W, Okhovat J, McPherson G, Burger S. Human-derived raw materials: controlled, consistent collection and cryopreservation enable successful manufacturing of cell-based products. Cytotherapy , Volume 16 , Issue 4 , S103, DOI: http://dx.doi.org/10.1016/j.jcyt.2014.01.380

Febg Q, Shabrani N, Thon JN, Huo H, Thiel A, Machlus KR, Kim K, Brooks J, Li F, Luo C, Kimbrel EA, Want J, Kim K, Italiano J, Cho J, Lu S, Lanza R. Scalable Generation of Universal Platelets from Human Induced Pluripotent Stem Cells, Stem Cell Reports (2014), http://dx.doi.org/10.1016/j.stemcr.2014.09.010

Banas R, Miller C, Guzik L, Zeevi A. Amnion-Derived Multipotent Progenitor Cells Inhibit Blood Monocyte Differentiation Into Mature Dendritic Cells. Cell Transplantation, Volume 23, Number 9, 2014, pp. 1111-1125, http://dx.doi.org/10.3727/096368913X670165

Badraiq H, Devito L, Ilic D. Isolation and Expansion of Mesenchymal Stromal/Stem Cells from Umbilical Cord Under Chemically Defined Conditions. Methods in Molecular Biology, DOI 10.1007/7651_2014_116, © Springer Science+Business Media New York 2014

Yee K, Malliaras K, Kanazawa H, Tseliou E, Cheng K, Luthringer DJ, Ho CS, Takayama K, Minamino N, Dawkins JF, Chowdhury S, Duong DT, Seinfeld J, Middleton RC, Kharmakumar R, Li D, Marban L, Makkar RR, Marban E. Allogeneic Cardiospheres Delivered via Percutaneous Transendocardial Injection Increase Viable Myocardium, Decrease Scar Size, and Attenuate Cardiac Dilatation in Porcine Ischemic Cardiomyopathy. PLoS ONE 9(12): e113805. doi:10.1371/journal.pone.0113805

White C, Benson K, Cherukuri P, Beals T. C-40: Cryobiology: Changing the face of regenerative medicine. Cryobiology; Volume 69, Issue 3, December 2014, Pages 511–512. doi:10.1016/j.cryobiol.2014.09.327

Clinton J, Shapiro B. Differentiation and expansion of hematopoietic precursor cells from bone-marrow derived CD34+ progenitors. (2014)  ATCC, AP Note 16

Niloufar Safinia, Giovanna Lombardi, Robert Lechler. An alliance with Miltenyi Biotec in the fight for tolerance: A venture into no man’s land. (2014). MACS & More, Vol 16, 1/2014, p. 13.

Senesac J, et al. (2014) Dendritic Cells Transfected with Adenoviral Vectors as Vaccines. In Michael J.P. Lawman and Patricia D. Lawman (eds.), Cancer Vaccines: Methods and Protocols, Methods in Molecular Biology, vol. 1139, DOI 10.1007/978-1-4939-0345-0_10, © Springer Science+Business Media New York 2014.

Kolodziej S, et al. PADI4 acts as a coactivator of Tal1 by counteracting repressive histone arginine methylation. Nat. Commun. 5:3995 doi: 10.1038/ncomms4995 (2014).

Michaud NR, Wang Y, McEachern K, et al. Novel neutralizing hedgehog antibody MEDI-5304 exhibits antitumor activity by inhibiting paracrine hedgehog signaling. Mol Cancer Ther Published OnlineFirst December 16, 2013. doi:10.1158/1535-7163.MCT-13-0420

Pei S, Minhajuddin M, Callahan KP, et al. Targeting Aberrant Glutathione Metabolism to Eradicate Human Acute Myelogenous Leukemia Cells. JBC Papers in Press. Published on October 2, 2013 as Manuscript M113.511170 doi: 10.1074/jbc.M113.511170.

Dannull J, Haley NR, Archer G, et al. Melanoma immunotherapy using mature DCs expressing the constitutive proteasome. J Clin Invest. 2013 July 1; 123(7): 3135–3145. Published online 2013 June 24. doi: 10.1172/JCI67544

Lagadinou ED, Sach A, Callahan K, Rossi RM, Neering SJ, et al. BCL-2 Inhibition Targets Oxidative Phosphorylation and Selectively Eradicates Quiescent Human Leukemia Stem Cells. Science Direct. (2013) Volume 12, Issue 3, 7 March 2013, pps 329-341. 2013 doi:10.1016/j.stem.2012.12.013

Nettenstroma L, Alderson K, Raschke EE, Evans MD, Sondel PM, Olek S, Seroogy SML. An Optimized Multi-parameter Flow Cytometry Protocol for Human T Regulatory Cell Analysis on Fresh and Viably Frozen Cells, Correlation with Epigenetic Analysis, and Comparison of Cord and Adult Blood. Published in final edited form as: J Immunol Methods. 2013 January 31; 387(1-2): 81–88. doi:10.1016/j.jim.2012.09.014.

Pattasseril J, Varadaraju H, Lock L , Rowley JA. Downstream Technology Landscape for Large-Scale Therapeutic Cell Processing. (2013) BioProcess International, Vol. 11(3)s 38-47.

Hamy F. Controlled-Rate Freezing of Cells During Ultra Cold Transit. (2013) Fisher BioServices, Part of Thermo Fisher Scientific. Found here: Fisher BioServices

Parrish NF. Phenotypic Characteristics of Mucosally Transmitted Hiv-1. Publicaly Accessible Penn Dissertations, Paper 684 (2013)

NIH. Single-cell-based non-colony type monolayer culture (NCM) of hESC. In Stem Cell Information [World Wide Web site]. Bethesda, MD: National Institutes of Health, U.S. Department of Health and Human Services, 2013 [cited Tuesday, November 04, 2014] Available at http://stemcells.nih.gov/research/nihresearch/scunit/pages/non_colony.aspx

Putnam AL, Safinia N, et al. Clinical Grade Manufacturing of Human Alloantigen-Reactive Regulatory T Cells for Use in Transplantation. American Journal of Transplantation 2013; XX: 1–11, Wiley Periodicals Inc. doi: 10.1111/ajt.12433

Zhou T, Benda C, Dunzinger S, Huang Y, Ho J.C, Yang J, Wang Y, Zhang Y, Zhuang Q, Li Y, Bao X, Tse H, Grillari J, Grillari-Voglauer R, Pei D, Esteban MA. Generation of human induced pluripotent stem cells from urine samples. Nature Protocols, 2012; Vol 7, No. 12, 280-289. Published online 8 November 2012; doi:10.1038/nprot.2012.115

Reynolds, Matthew R. ; Weiler, Andrea M.; Piaskowski, Shari M.; et al. A trivalent recombinant Ad5 gag/pol/nef vaccine fails to protect rhesus macaques from infection or control virus replication after a limiting-dose heterologous SIV challenge. Vaccine. 2012 June 22; 30(30): 4465–4475. doi:10.1016/j.vaccine.2012.04.082.

 

Gülen D, Maas S, Julius H, Warkentin P, Britton H, Younos I, Senesac J, Pirruccello SM, Talmadge JE. Cryopreservation of adenovirus-transfected dendritic cells (DCs) for clinical use. Int Immunopharmacol. 2012 May;13(1):61-8. doi: 10.1016/j.intimp.2012.03.009. Epub 2012 Mar 27.

Zhou Y, Fowler Z, Cheng A, Sever R. Improve Process Uniformity and Cell Viability in Cryopreservation. (2012) BioProcess International 1 Apr 2012.

Buffington DA, Pino CJ, Chen L, Westover AJ, Hageman G, Humes HD. Bioartificial Renal Epithelial Cell System (BRECS): A Compact, Cryopreservable Extracorporeal Renal Replacement Device. Cell Medicine, Vol. 4, pp. 33–43, 2012 2155-1790/12 $90.00. Printed in the USA. All rights reserved. DOI: http://dx.doi.org/10.3727/215517912X653328.

Frisch BJ, Ashton JM, Xing L , Becker MW, Jordan CT, Calvi LM. Functional inhibition of osteoblastic cells in an in vivo mouse model of myeloid leukemia. Published online before print September 28, 2011, doi: 10.1182/blood-2011-04-348151 Blood January 12, 2012 vol. 119 no. 2 540-550

Tobias Hofving. CD133 and ALDH1 as Prospective Markers for Cancer Stem Cells in Ileal Carcinoids

Tannenbaum SE, Tako Turetsky T, Singer O, Aizenman E, Kirshberg S, et al. Derivation of Xeno-Free and GMP-Grade Human Embryonic Stem Cells – Platforms for Future Clinical Applications. PLoS ONE 7(6): e35325. doi:10.1371/journal.pone.0035325(2012)

Turner R, Mendel G, Wauthier E, Barbier C, Reid LM. Hyaluronan-Supplemented Buffers Preserve Adhesion Mechanisms Facilitating Cryopreservation of Human Hepatic Stem/Progenitor Cells. CT-0574 Cell Transplantation Epub, provisional acceptance. DOI: 10.3727/096368912X637000

Tran C, Torres-Coronado M, Gardner A, Gu A, Vu H, Rao A, Cao IF, Ahmed A, Digiusto D. Optimized Processing of Growth Factor Mobilized Peripheral Blood CD34+ Products by Counterflow Centrifugal Elutriation. Stem Cells Translational Medicine 2012;1:422–429.

 

Alexandre E, Baze A, Parmentier C, Desbans C, Pekthong D, Gerin B, Wack C, Bachellier P, Heyd B, Weber J, Richert L. Plateable cryopreserved human hepatocytes for the assessment of cytochrome P450 inducibility: experimental condition-related variables affecting their response to inducers. Xenobiotica, 2012; Early Online: 1–12. DOI: 10.3109/00498254.2012.676693

Li Y, Ma T. Bioprocessing of Cryopreservation for Large-Scale Banking of Human Pluripotent Stem Cells. BioResearch Open AccessVolume XX, Number XX, Month 2012, Mary Ann Liebert, Inc. DOI: 10.1089/biores.2012.0224

Wang X, Naranjo A, Brown CE, Bautista C, Wong CW, Chang W, Aguilar B, Ostberg JR, Riddell SR, Forman SJ, Jensen MC. Phenotypic and Functional Attributes of Lentivirus-modified CD19-specific Human CD8+ Central Memory T Cells Manufactured at Clinical Scale. Journal of Immunotherapy: November–December 2012; Volume 35, Issue 9, p 689–701, doi: 10.1097/CJI.0b013e318270dec7.

Jin S, Yao H, Weber JL, Melkoumian ZK, Ye K. A Synthetic, Xeno-Free Peptide Surface for Expansion and Directed Differentiation of Human Induced Pluripotent Stem Cells. PLoS ONE 7(11): e50880. doi:10.1371/journal.pone.0050880. (November 2012)

Rios M. Optimizing Cryopreservation for Therapeutic Cells. BioProcess International, Vol. 9, No. 4, April 2011, pp. 26–30

Fritsch G, Witt V, Matthes S, Dworzak M, Artwohl M, Clarke D, Mathew AJ. Improved Post-Thaw Stability Validation of Peripheral Blood Cell Products Utilizing the Intracellular-Like Cryostor Cryopreservation Solution, and Preliminary Results of Clinical Application. Biology of Blood and Marrow Transplantation. February 2011; Volume 17, Issue 2, S210-S211.

Woods EJ, Thirumala S. Packaging Considerations for Biopreservation Transfusion Medicine and Hemotherapy. 2011; 38(2): 149–156.

Nicoud IA, Clarke DM, Taber G, Stolowski KM, Roberge SE, Song MK, Mathew AJ, Reems J. Cryopreservation of umbilical cord blood with a novel freezing solution that mimics intracellular ionic composition. Transfusion. doi: 10.1111/j.1537-2995.2011.03547.x

Xu C, Police S, Hassanipour M, et al. Efficient generation and cryopreservation of cardiomyocytes derived from human embryonic stem cells. Regen Med. 2011 Jan;6(1):53-66. doi: 10.2217/rme.10.91.

Lai-Hipp C, Goldberg T, Scott E, Ziman A, Vyas G. Pooled peripheral blood mononuclear cells provide an optimized cellular substrate for human immunodeficiency virus Type 1 isolation during acute infection. Transfusion 51: 333-337 (February 2011)

Engelhardt BG, Jagasia M, Savani BN, Bratcher NL, et at.  Regulatory T cell expression of CLA or α4β7 and skin or gut acute GVHD outcomes Bone Marrow Transplantation (2011) 46, 436–442; doi:10.1038/bmt.2010.127; published online 31 May 2010.

Murphy S, Rosli S, Acharya R, Mathias L, Lim R, Wallace E, Jenkin G. Amnion Epithelial Cell Isolation and Characterization for Clinical Use. Current Protocols in Stem Cell Biology. June, 2010. UNIT 1E.6.

Malpique R, Osório LM, Ferreira DS, Ehrhart F, Brito C, Zimmermann H, Alves PM. Alginate Encapsulation as a Novel Strategy for the Cryopreservation of Neurospheres. Tissue Eng Part C Methods. 2010 Feb 8.

Sivarajah S, Raj GS, Mathews AJ, Sahib NB, Hwang WS, Crook JM.The generation of GLP-grade human embryonic stem cell banks from four clinical-grade cell lines for preclinical research. In Vitro Cell Dev Biol Anim. 2010 Apr;46(3-4):210-6. doi: 10.1007/s11626-010-9287-1. Epub 2010 Feb 23.

Mathew A.J. I’m Losing Cell Viability and Function at Different Points in My Process, and I Don’t Know Why! BioProcess International, 8 (6): 54-7 (2010).

Kim AJ, Adkisson HD, Wendland M, Seyedin M, Berven S, Lotz JC. Juvenile Chondorcytes May Facilitate Disc Repair (2010) The Open Tissue Engineering and Regenerative Medicine Journal, 2010, 3, 28-35.

Hessel A, Schwendinger M, Fritz D, Coulibaly S, Holzer GW, et al.  A Pandemic Influenza H1N1 Live Vaccine Based on Modified Vaccinia Ankara Is Highly Immunogenic and Protects Mice in Active and Passive Immunizations. (2010) PLoS ONE 5(8): e12217. doi:10.1371/journal.pone.0012217.

Valentine LE, Loffredo JT, Bean AT, León EJ, MacNair CE, Beal DR, Piaskowski SM, Klimentidis YC, Lank SM, Wiseman RW, Weinfurter JT, May GE, Rakasz EG, Wilson NA, Friedrich TC , O’Connor DH, Allison DB, Watkins DI. Infection with “Escaped” Virus Variants Impairs Control of Simian Immunodeficiency Virus SIVmac239. Journal of Virology, November 2009, p. 11514-11527, Vol. 83, No. 22. Copyright © 2009, American Society for Microbiology.

Luckett-Chastain LR, Gallucci RM. Interleukin (IL)-6 Modulates Transforming Growth Factor-β Expression in Skin and Dermal Fibroblasts from IL-6-Deficient Mice. Br J Dermatol. 2009 August; 161(2): 237–248.

Güll I, Alves PM, Gabor F, Wirth M. Viability of the Human Adenocarcinoma Cell Line Caco-2: Influence of Cryoprotectant, Freezing Rate, and Storage Temperature. Sci Pharm. 2009; 77: 133–141.

Malpique R, Ehrhart F, Katsen-Globa A, Zimmermann H, Alves PM. Cryopreservation of Adherent Cells: Strategies to Improve Cell Viability and Function after Thawing. Tissue Engineering: Part C, Volume 15, Number 00, 2009.

Thirumala S, Goebel WS, Woods EJ. Clinical Grade Adult Stem Cell Banking. Organogenesis. 2009 Jul–Sep; 5(3): 143–154. © 2009 Landes Bioscience.

Woods EJ, et al. Optimized cryopreservation method for human dental pulp-derived stem cells and their tissues of origin for banking and clinical use. Cryobiology. 2009 Oct;59(2):150-7.

Perry BC, Zhou D, Wu X, Yang FC, Byers MA, Chu G, Hockema JJ, Woods EJ, Goebel WS. Collection, Cryopreservation, and Characterization of Human Dental Pulp–Derived Mesenchymal Stem Cells for Banking and Clinical Use. Tissue Engineering Part C: Methods. June 2008, 14(2): 149-156. doi:10.1089/ten.tec.2008.0031.

Ennis J, Sarugaser R, Gomez A, Baksh D, Davies JE. Isolation, Characterization, and Diverentiation of Human Umbilical Cord Perivascular Cells (HUCPVCs). Methods in Biology, Vol. 86.

Crook JM, Peura TT, Kravets L, Bosman AG, Buzzard JJ, Horne R, Hentze H, Colman A. The Generation of Six Clinical-Grade Human Embryonic Stem Cell Lines. Cell Stem Cell, Volume 1, Issue 5, 490-494, 15 November 2007 doi:10.1016/j.stem.2007.10.004

Baust JM, Snyder KK, Mathew, AJ, Van Buskirk RG., Baust, John G. Steps to Improving Cryopreservation Outcome: Understanding key factors influencing efficacy.Bioscience Technology (2006)

Corning. Enhanced Survival of LNCaP Cells Following Cryopreservation Using the CryoStor™ CS5 Preservation Solution and Corning® CellBIND® Surface (2006)

Stylianou J, Vowels M, Hadfield K. Novel Cryoprotectant Significantly Improves the Post-thaw Recovery and Quality of HSC from CB. Cytotherapy, 8(Suppl 1):57. (2006).

Robilotto AT, Baust JM, Van Buskirk R, Baust JG. Involvement of the cysteine protease calpain family in the cell death following cryopreservation. Cell Preservation Technology 4(1) 2006.

Baust JM. Advances in Media for Cryopreservation and Hypothermic Storage. BioProcess International Supplement June: 46-56 (2005).

Van Buskirk RG, Snyder KK, Baust JG, Mathew AJ, Baust JM. Cryopreservation – It’s Not Just About Cell Yield. BioProcessing International,  April (2005).

Sosef MN, Baust JM, Sugimachi K., Fowler A, Tompkins RG, Toner M. Cryopreservation of isolated primary rat hepatocytes: enhanced survival and long-term hepatospecific function. Ann Surgery 241(1):125-33 (2005).

Snyder KK, Van Buskirk RG, Mathew AJ, Baust JG, Baust JM. Biological Packaging for the Global Cell and Tissue Therapy Markets. BioProcessing Journal May/June: 39-46 (2004).

Cho JJ, Joseph B, Sappal BS, Giri RK, Wang R, Ludlow JW, Furth ME, Susick R, Gupta S. Analysis of the functional integrity of cryopreserved human liver cells including xenografting in immunodeficient mice to address suitability for clinical applications. Liver Int. 24(4):361-70 (2004).

Sugimachi K., Sosef MN, Baust JM, Fowler A, Tompkins RG, Toner M. Long-Term Function of Cryopreserved Rat Hepatocytes in a Coculture System. Cell Transplantation 13: 187-195 (2004).

Baust JM. Molecular mechanisms of cellular demise associated with cryopreservation failure. Cell Preservation Technology 1(1): 17-31 (2002)

Baust JM, Van Buskirk RG, Baust JG. Gene activation of the apoptotic caspase cascade following cryogenic storage. Cell Preservation Technology 1(1): 63-80 (2002)

Baust JM, Van Buskirk RG, Baust JG. Modulation of the cryopreservation cap: Elevated survival with reduced dimethyl sulfoxide concentration. Cryobiology 45: 97-108 (2002).

Baust JM, Vogel MJ, Van Buskirk RG, Baust JG. A molecular basis of cryopreservation failure and its modulation to improve cell survival. Cell Transplantation 10(7): 561-71 (2001).

Lakey JR, Rajotte RV, Fedorow CA, Taylor MJ. Islet cryopreservation using intracellular preservation solutions. Cell Transplantation 10(7): 583-9 (2001).

Baust JM, Van Buskirk RG, Baust JG. Cell viability improves following inhibition of cryopreservation-induced apoptosis. In Vitro Cellular and Developmental Biology, 36(4): 262-70 (2000).

Baust JM, Van Buskirk RG, Baust JG. Cryopreservation of an engineered skin equivalent: The apoptosis paradigm. Journal of the American Society of Mechanical Engineers (Advances in Heat and Mass Transfer in Biotechnology): HTD 363: 71-6 (1999).

Gramlich OW, Zhu W, Burand A, Ankrum JA, et al. Transplantation of cryopreserved MSC rescues retinal ganglion cells following I/R injury. ARVO 2016 Annual Meeting Abstracts (2016).

Fearnot ER, Fazekasova H, Thirkell S, Lowe K, Bushell A, Lombardi G. C-25: Evaluation of closed system medical device for low volume storage for clinical studies involving regulatory T cells. Cryobiology (2014). Volume 69, Issue 3, December 2014. Pages 508. doi:10.1016/j.cryobiol.2014.09.312

Parrish NF, Gao F, Li H, Giorgi EE, Barbian HJ, Parrish EH, Zajic L, et al. Phenotypic properties of transmitted founder HIV-1 [published online before print March 29, 2013]. PNAS April 23, 2013 vol. 110, no. 17 6626-6633 doi:10.1073/pnas.1304288110.

Gülen D, Maas S, Julius H, Warkentin P, Britton H, Younos I, Senesac J, Pirruccello SM, Talmadge JE. Cryopreservation of adenovirus-transfected dendritic cells (DCs) for clinical use. Int Immunopharmacol. 2012 May;13(1):61-8. Epub 2012 Mar 27.

Pino CJ, Yevzlin AS, Lee K, Westover AJ, Smith PL,Buffington DA, Humes HD. Cell-based approaches for the treatment of systemic inflammation. Nephrol Dial Transplant (2012) 1–7. doi: 10.1093/ndt/gfs503

Yasuhara T, Matsukawa N, Hara K, Maki M, Ali MM, Yu SJ, Bae E, Yu G, Xu L, McGrogan M, Bankiewicz K, Case C, Borlongan CV. Notch-Induced Rat and Human Bone Marrow Stromal Cell Grafts Reduce Ischemic Cell Loss and Ameliorate Behavioral Deficits in Chronic Stroke Animals. Stem Cells and Development. December 2009, 18(10): 1501-1514. doi:10.1089/scd.2009.0011.

Baust JM, Kulkarni H, Vogel MJ, Van Buskirk RG, Baust JG. Cellular Proteomic Alterations Following Cryopreservation Influence Cell Survival. Cryobiology, Vol. 51 No. 4, pg. 406, 2005.

ISCT 2016 Brooks-BioLife Poster 5-19-16 Printed VersionNext Generation Technology Procedures and Products Facilitate Biopreservation Best Practices and Increased Viability for Cellular Therapy

BioLife-UW ISCT poster final 5-17-16Optimized cryopreservation of clinically-relevant cell banks using a GMP, chemically defined, xeno-free media

ISCT-Poster_Whitepaper-Cytotherapy-2016Off the shelf cellular therapeutics: Factors to consider during cryopreservation and storage of human cells for clinical use.
 

MasthercellPosterMai2015A0WEBTechnology Transfer and Process Development for an Autologous Cell Therapy Against Multiple Sclerosis

 

Pub_2-Maxey_CLT-008_2014Immuno-Protective and Immuno-Suppressive Behavior Displayed by a Novel Viable Cell Bone Matrix

Pub_2-Maxey_CLT-008_2014Development of a Mouse Myeloid Progenitor Cell Therapeutic for Treatment of HS-ARS in BALB/c mice

AABBPoster101815.Final Identification of CD73, CD90 and CD105 by qPCR in Cryopreserved Human Cord Tissue

Scalability of Allogeneic Stem Cell Manufacturing

Development of a quantitative measurement of mesenchymal stem cells explant outgrowth from umbilical cord tissue

ISCT Poster FvT 052115 FINAL_roosterbio_icon Cryopreserved hMSCsmaintain comparable in vitrofunctional activity compared to fresh hMSCs

ISCT Poster 2015_FINAL TCH HemaCare Cryopreserved Leukopaks Maintain Cell Viability and Functionality: A Solution for Cell Therapy Logistics

2014 BEST 65 POSTER Cryopreservation of donor lymphocyte clinical products with conventional 10% DMSO and CryoStor®

Optimisation of cGMP Cryopreservation of hESC using CryoStor® Part 1

Optimisation of cGMP Cryopreservation of hESC-Roslin Cells Optimisation of cGMP Cryopreservation of hESC using CryoStor® Part 2

Poster_Eyrich_ISCT2013_135 GMP-Compliant Production Dendritic Cells

2013CBForum poster abicon Reduced Engraftment Times

IBET_cryopreservation of cardiomyocytes ECI_Page_1 Cryopreservation Of Cardiomyocytes

Effect of Cryopreservation on Cell Viability, Recovery, and Proliferation

Stem Cell Cryopreservation Stability

Gel Improves Stability and Recovery

Cord-Blood-Stem-Cell-Banking_thumb Cord Blood Stem Cell Banking Using CryoStor

Maximizing-Stability-of-Human-Stem-Cells.png Cryopreservation of Adherent Cells Using CryoStor

Cryopreservation Strategies of Adherent Cells

Improving Cryopreservation Yield

Safety Study of Intravenous Injection of Solutions

Accelerated Stability Study

Mitochondrial-Associated Pathway of Apoptosis Contributes to Failure

Cellular Proteomic Alterations Influence Cell Survival

Calpain Activation Contributes to Cell Death

Evolution of Biological Packaging and Biopreservation

Cryopreservation of Hepatocytes

Improved Stability Validation of Peripheral Blood Cell Products

Standardized Drug-Testing Using Tumor Samples

Considerations for cGMP Production of Patient-Specific Cell Therapeutics – A PACT Case Study. Derek JH. Webinar(slides). (2014).

Hypothermic Storage & Shipping Media

Henry TD, Schaer GL, DeMaria A, Recker D, Remmers AE, Goodrich J, Patel A.  The iXCELL-DCM Trial: Rationale and Design.  Cell Transplant. 2016 Mar 22. [Epub ahead of print]. DOI: 10.3727/096368916X691295

Blázquez R, Sánchez-Margallo FM, Crisóstomo V, Báez C, Maestre J, García-Lindo M, et al. (2015) Intrapericardial Administration of Mesenchymal Stem Cells in a Large Animal Model: A Bio-Distribution Analysis. PLoS ONE 10(3): e0122377. DOI:10.1371/journal.pone.0122377

Anderson MJ, David ML, Scholz M, Bull SJ, Morse D, Stevens MH, Peterson ML. Efficacy of Skin and Nasal Povidone-Iodine Preparation against mupirocin resistant MRSA and Staphylococcus aureus within the anterior nares. AAC Accepted Manuscript Posted Online 2 March 2015 Antimicrob. Agents Chemother. DOI:10.1128/AAC.04624-14

AlMulhem, N.A. (2015). Cryopreservation and Hypothermal Storage of Hematopoietic Stem Cells (Master Thesis). Retrieved from https://etd.ohiolink.edu/!etd.send_file?accession=ucin1439307244&disposition=attachment

AGE Day, Bhangra KS, Murray-Dunning C, Thanabalasundaram L, Grace N, Cameron G, Stevanato L, Sinden J, Phillips JB. Hypothermic and cryogenic preservation of artificial neural tissue made using differentiated CTX human neural stem cells in collagen gels. European Cells and Materials. Vol 29. Suppl. 3, (2015). Pg 44.

Carver GE, Locknar SA, Morrison WA, Ramanujan VK, Farkas DL. High-speed multispectral confocal biomedical imaging. Journal of Biomedical Optics 19(3), 036016 (March 2014). DOI: 10.1117/1.JBO.19.3.036016

Bartunek J, Behfar A, Dolatabadi D, Vanderheyden M, Ostojic M, Dens J, et al. Cardiopoietic Stem Cell Therapy in Heart Failure. Journal of the American College of Cardiology Vol. 61, No. 23, 2013, 0735-1097.

Usta OB, Kim Y, Ozer S, Bruinsma BG, Lee J, Demir E, et al. (2013) Supercooling as a Viable Non-Freezing Cell Preservation Method of Rat Hepatocytes. PLoS ONE 8(7): e69334. doi:10.1371/journal.pone.0069334

Campbell SD, Crafford A, Williamson BL, Kharasch ED. Mechanism of Autoinduction of Methadone N-Demethylation in Human Hepatocytes. Published online before print June 3, 2013, doi: 10.1213/​ANE.0b013e3182918252. A & A July 2013 vol. 117 no. 1 52-60

Randelli P, Conforti E, Piccoli M, Ragone V, Creo P, Cirillo F, Masuzzo P, Tringali C, Cabitza P, Tettamanti G, Gagliano N, Anastasia L. Isolation and Characterization of 2 New Human Rotator Cuff and Long Head of Biceps Tendon Cells Possessing Stem Cell–Like Self-Renewal and Multipotential Differentiation Capacity. The American Journal of Sports Medicine. 2013. DOI: 10.1177/0363546512473572 2013.

Mathew AJ. A review of cellular biopreservation considerations during hair transplantation. Hair Transplant Forum International, 2013; 23(1):1-11.

Smith EJ, Stroemer RP, Gorenkova N, Nakajima M, Crum WR, Tang E, Stevanato L, Sinden JD, Modo M. Implantation Site and Lesion Topology Determine Efficacy of a Human Neural Stem Cell Line in a Rat Model of Chronic Stroke. Published online before print: 22 MAR 2012 DOI: 10.1002/stem.1024. Stem Cells Volume 30, Issue 4, pages 785–796, April 2012.

Spitzer TL, Rojas A, Zelenko Z, Aghajanova L, Erikson DW, Barragan F, Meyer M, Tamaresis JS, Hamilton AE, Irwin JC, Giudice LC. Perivascular Human Endometrial Mesenchymal Stem Cells Express Pathways Relevant to Self-Renewal, Lineage Specification, and Functional Phenotype. Biology of Reproduction. 2012 Feb 29; 86(2):58. Published 9 November 2011. Print 2012 Feb.; DOI:10.1095/biolreprod.111.095885.

Ginis I, Grinblat B, Shirvan MH. Evaluation of Bone Marrow-Derived MSC after Cryopreservation and Hypothermic Storage in Clinically Safe Medium. Tissue Engineering Part C: Methods. (2012) Volume 18, Number 6, 2012. DOI: 10.1089/ten.2011.0395.

Akabawy GE, Rattray I, Johansson SM, Gale R, Bates G, Modo M. Implantation of undifferentiated and predifferentiated human neural stem cells in the R6/2 transgenic mouse model of Huntington’s disease. BMC Neuroscience (2012), 13:97; DOI:10.1186/1471-2202-13-97.

Povsic TJ, O’Connor CM, Henry T, Taussig A, et al. A double-blind, randomized, controlled, multicenter study to assess the safety and cardiovascular effects of skeletal myoblast implantation by catheter delivery in patients with chronic heart failure after myocardial infarction. American Heart Journal, October 2011. Volume 162, Number 4, 654-662. DOI: http://dx.doi.org/10.1016/j.ahj.2011.07.020.

Powell RJ, Comerota AJ, Berceli SA, Guzman R, Henry TD, Tzeng E, Velazquez O, Marston WA, Bartel RL, Longcore A, Stern T, Watling S. Interim analysis results from the RESTORE-CLI, a randomized, double-blind multicenter phase II trial comparing expanded autologous bone marrow-derived tissue repair cells and placebo in patients with critical limb ischemia. Journal of Vascular Surgery. (2011) Oct;54(4):1032-41.

Woods EJ, Thirumala S. Packaging Considerations for Biopreservation. Transfusion Medicine and Hemotherapy. 2011; 38(2): 149–156. DOI: 10.1159/000326083

Lowe NJ, Lowe PL, St. Clair Roberts J. Phase IIa Open-Label Dose-Escalation Pilot Study Using Allogeneic Human Dermal Fibroblasts for Nasolabial Folds. Dermatologic Surgery. (2010). Volume 36, Issue 10, October 2010, Pages: 1578–1585. Epub 2010 Aug 16. DOI:10.1111/j.1524-4725.2010.01686.x.

Nève N, Kohles SS, Winn SR, Tretheway DC. Manipulation of Suspended Single Cells by Microfluidics and Optical Tweezers. (2010). Cellular and Molecular Bioengineering. Vol 3, No 3, 213-228. DOI:10.1007/s12195-010-0113-3.

Mathew, AJ.  I’m Losing Cell Viability and Function at Different Points in My Process, and I Don’t Know Why! BioProcess International, 8 (6): 54-7 (2010).

Yang Y, Steeg J, Honaramooz A. The effects of tissue sample size and media on short-term hypothermic preservation of porcine testis tissue. (2010). Cell and Tissue Research. 2010 May;340(2):397-406. Epub 2010 Mar 16.

Parsley WM, Perez-Meza D. Review of Factors Affecting the Growth and Survival of Follicular Grafts. (2010). J Cutan Aesthet Surg. 2010 May–Aug; 3(2): 69–75. DOI:10.4103/0974-2077.69014.

Stéphenne X, Najimi M, Sokal EM. . Hepatocyte Cryopreservation: Is it Time to Change the Strategy? (2010). World Journal of Gastroenterology. 2010 January 7; 16(1): 1-14.

Ostrowska A, Gu K, Bode DC, Van Buskirk RG. Hypothermic storage of isolated human hepatocytes: a comparison between University of Wisconsin solution and a hypothermosol platform. Archives of Toxicology. 2009 May;83(5):493-502.

Perry BC, Zhou D, Wu X, Yang FC, Byers MA, Chu TMG, Hockema JJ, Woods EJ, Goebel WS. Collection, Cryopreservation, and Characterization of Human Dental Pulp–Derived Mesenchymal Stem Cells for Banking and Clinical Use. Tissue Engineering Part C: Methods. June 10, 2008, 14(2): 149-156. DOI:10.1089/ten.tec.2008.0031.

Van Buskirk RG, Snyder KK, Mathew AJ, Baust JG, Baust JM. Navigating the Postpreservation Viability Fog. (2006). GEN. Nov 1, 2006 (Vol. 26, No. 19)

Baust, JM. Advances in Media for Cryopreservation and Hypothermic Storage. BioProcess International Supplement, June: 46-56 (2005).

Snyder KK, Van Buskirk RG, Baust JM, Mathew AJ, Baust JG. Enhanced Hypothermic Storage of Neonatal Cardiomyocytes. Cell Preservation Technology 3(1):61-74 (2005).

Snyder, K.K., Van Buskirk, R.G., Baust, J.M., Mathew, A.J., Baust, J.G. Biological Packaging for the Global Cell and Tissue Therapy Markets. BioProcessing Journal May/June: 39-46 (2004).

Baust JM, Mathew AJ, Snyder K, Liu E, Van Buskirk RG, Hardy M, Baust JG. Transplantation Diagnostics: A Preliminary Analysis Using Protein Microarray to Determine Kidney Status Prior To and Following Implantation. Cell Preservation Technology 2(2): 81-90 (2004).

Bessems M, Doorschodt BM, van Vliet AK, van Gulik TM. Preservation of rat livers by cold storage: a comparison between the University of Wisconsin solution and HypoThermosol. Ann Transplant 9(2): 35-7 (2004).

Mathew AJ, Baust JM, Van Buskirk RG, Baust JG. Cell Preservation in Reparative and Regenerative Medicine: Evolution of Individualized Solution Composition. Tissue Engineering 10(11/12): 1662-1671 (2004).

Van Buskirk RG, Baust JM, Snyder KK, Mathew AJ, Baust JG. . Hypothermic Storage and Cryopreservation – The Issues of Successful Short-term and Long-term preservation of cells and tissues. Bioprocessing International 2(10): 42-49 (2004).

Mathew AJ, Van Buskirk RG, Baust JG. Improved Hypothermic Preservation of Human Renal Cells Through Suppression of Both Apoptosis and Necrosis. Cell Preservation Technology 1(4):239-251 (2003).

Ikonomovic M, Kelly KM, Hentosz TM, Shih SR, Armstrong DM, Taylor MJ. Ultraprofound cerebral hypothermia and blood substitution with an acellular synthetic solution maintains neuronal viability in rat hippocampus. CryoLetters 22: 19-26 (2001).

Dahdah NS, Taylor MJ, Russo P, Wagerle LC. . Effects of HypoThermosol, an experimental acellular solution for tissue preservation and cardiopulmonary bypass, on isolated newborn lamb coronary vessels subjected to ultra profound hypothermia and anoxia. Cryobiology, 39, 58-68 (1999).

Mathew AJ, Hollister WR, Addona T, Baust JG, Van Buskirk RG. Vitamin E and EDTA improve the efficacy of HypoThermosol – implication of apoptosis. In Vitro and Molecular Toxicology 12(3): 163-72 (1999).

Mathew AJ, Baust JG, Van Buskirk RG. Optimization of hypothermosol for the hypothermic storage of cardiac cells – addition of EDTA. In Vitro Toxicology. 10(4): 407-415, Winter. (1997).

Van Buskirk RG, Rauch J, Robert S, Taylor MJ, Baust JG. Assessment of hypothermic storage of normal human epidermal keratinocytes (NHEK) using alamar blue. In Vitro Toxicology, 9(3), 297-303 (1996).

Simon D, Taylor MJ, Elrifai AM, et al. Hypothermic blood substitution enables resuscitation after hemorrhagic shock and 2 hours of cardiac arrest. ASAIO J. 1995 Jul-Sep;41(3):M297-300.

Taylor MJ, Bailes JE, Elrifai AM, Shih SR, Teeple E, Leavitt ML, Baust JG, Maroon JC. A New Solution for Life Without Blood: Asanguineous Low-Flow Perfusion of a Whole-Body Perfusate During 3 Hours of Cardiac Arrest and Profound Hypothermia. Circulation 91(2): 431-44 (1995).

Cook JR, Eichelberger H, Robert S, Rauch J, Baust JG, Taylor MJ, Van Buskirk RG. Cold-storage of synthetic human epidermis in HypoThermosol. Tissue Engineering 1(4): 361-77 (1995).

Rhoads LS. Use of Hypothermically Stored Keratinocyte Cultures for In Vitro Toxicology. Journal of Toxicology: Cutaneous and Ocular Toxicology. 13(3): 231-48 (1994).

Herias M. Investigation of HypoThermosol® as a delivery medium for cardiomyocyte progenitor cellsCytotherapy 16(4):S38 (2014). DOI: http://dx.doi.org/10.1016/j.jcyt.2014.01.130

Snyder KK, Baust JM, Van Buskirk RG, Baust JG. Improved Hypothermic Storage of Sensitive Primary Cell Systems. Cytotherapy, Vol. 7 Supp. 1, pg. 2, 2005.

Snyder KK, Baust JM, Van Buskirk RG, Baust JG. Assessment of Function and Metabolic Activity of Neonatal Cardiomyocytes Following Hypothermic Storage. Cryobiology, Vol. 47 No. 3, pg. 273, 2003. (P.L. Steponkus Crystal Award Recipient)

Mathew AJ, Baust JG, Van Buskirk RG. Enhanced hypothermic preservation of multiple human cell types. Cryobiology 43:383 (2001).

Mathew AJ, Baust JG, Van Buskirk RG. Enhanced hypothermic preservation of human renal cells. Cryobiology 43:357 (2001).

Mathew, AJ, Baust JM, Hollister WR, Al-Abdullah IH, Ricordi C, Van Buskirk RG, Baust JG. Molecular biological analysis of cell death leads to improvement in the hypothermic solution, HypoThermosol. Cryobiology 41:335-6 (2000).

Mathew AJ, Hollister WR, Baust JG, Van Buskirk RG. Continued optimization of the cold storage solution HypoThermosol and implications of apoptosis. Cryobiology 37:398-9 (1998).

Wagerle LC, Taylor MJ, Dahdah NS, Russo P.   Effects of ultraprofound hypothermia on the ex vivo contractile response of cerebral arteries: Relevance to cerebrovascular dysfunction during cardiopulmonary bypass. Cryobiology 35:346 (1997).

Taylor MJ, Snyder M, Machen L, Clark RE, Baust JG. Functional survival of isolated rabbit hearts after cold storage in HypoThermosol modified with compounds that might promote retention and repletion of high energy phosphates. Cryobiology 33:620-1, (1996).

Elrifai AM, Taylor MJ, Shih SR, Bailes JE, Kiya N, Wilberger JE, Maroon JC.  Hypothermic preservation of immature spinal cord using HypoThermosol for transplantation in the rat. Cryobiology 33:675 (1996).

Van Buskirk RG, Taylor MJ, Shen R, Tang W, Mathew AJ, Baust JG. Optimization of HypoThermosol using cultured cardiomyocytes and the fluorescent multiple endpoint assay. Cryobiology 32:590 (1995).

Van Buskirk RG, Taylor MJ, Rauch J, Roberts S, Baust JG. Preservation of synthetic human skin in HypoThermosol. Cryobiology 32:591 (1995).

Taylor MJ, Clark RE, Snyder M, Machen L, Baust JG. Static cold storage of isolated rabbit hearts in HypoThermosol: Influence of butanedione monoxime during short-term storage. Cryobiology 32:538-9 (1995).

Taylor MJ, Clark RE, Baust JG. The importance of adenosine and glutathione as components of a new hypothermic blood substitute (HypoThermosol): Evaluation of myocardial protection at 7°C using an isolated working rabbit heart model. Cryobiology 30:593-4 (1994).

Taylor MJ, Simon D, Elrifai AM, Shih SR, Bailes JE, Baust JG, Maroon JC, Diamond DL. A feasibility study in a canine model for using profound hypothermia and blood substitution with HypoThermosol to enable resuscitation after hemorrhagic shock. Cryobiology 31:592-3 (1994).