After five days in the cold, do your cells look like those on the left, or those stored in HypoThermosol?

Biopreserved in Home-Brew Media

Biopreserved in HypoThermosol

Over 365 Articles, Abstracts, and Posters Citing the Biopreservation Efficacy of CryoStor and HypoThermosol.

Will CryoStor freeze media and HypoThermosol storage media preserve cells, tissues, and organs better than your alternatives?

We have dozens of studies, tests, and reviews that provides evidence that BioLife products outperform commercially available alternatives and ‘home-brew’ biopreservation media.

If you want to extend cell stability, improve cell viability, and lower the average cost per delivered dose of your cell therapy product (arguably the most important metric once safety and efficacy are proven), read on or ask one of our scientists directly.

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Cryopreservation Freeze Media

Jung, J., Buisman, S. C., Weersing, E., Dethmers-Ausema, A., Zwart, E., Schepers, H., Dekker, M. R., Lazare, S. S., Hammerl, F., Skokova, Y., Kooistra, S. M., Klauke, K., Poot, R. A., Bystrykh, L. V., de Haan, G. CBX7 Induces Self-Renewal of Human Normal and Malignant Hematopoietic Stem and Progenitor Cells by Canonical and Non-canonical Interactions. Cell Reports. 2019; 26(7), 1906-1918.e8. doi:10.1016/j.celrep.2019.01.050

Stroncek, D., Mathew, A., & McKenna, D. Cell therapies for trauma and critical care medicine: critical issues in translation for cellular and novel therapies in trauma and critical care. Transfusion. 2019; 59(S1), 854-857. doi:10.1111/trf.14832

Marín Morales, J. M., Münch, N., Peter, K., Freund, D., Oelschlägel, U., Hölig, K., Böhm, T., Flach, A., Keßler, J., Bonifacio, E., Bornhäuser, M., Fuchs, A.  Automated Clinical Grade Expansion of Regulatory T Cells in a Fully Closed System. Frontiers In Immunology. 2019; 10:38.  doi:10.3389/fimmu.2019.00038

Kim, M., Audet, J.   On-demand serum-free media formulations for human hematopoietic cell expansion using a high dimensional search algorithm.  Communications Biology. 2019; 2:48. doi:10.1038/s42003-019-0296-7

Mitsunaga, S., Shioda, K., Isselbacher, K. J., Hanna, J. H., Shioda, T.  Generation of Human Primordial Germ Cell-like Cells at the Surface of Embryoid Bodies from Primed-pluripotency Induced Pluripotent Stem Cells.  J. Vis. Exp. 2019; (143), e58297.  doi:10.3791/58297 (2019).

Kozbial, A., Weinstein, H., Murthy, S. K., Rossi, J. M.  Scale-up of a perfusion-based dendritic cell generation process.  Cell Gene Therapy Insights 2018; 4(11), 1117-1130.  doi: 10.18609/cgti.2018.110

Bai, X., Yang, X., Cheng, Y., Chen, L.  Establishment of an induced pluripotent stem cell line (FDEENTi001-A) from a patient with pathological myopia.  Stem Cell Research. 2019; 34, 101369.  doi:10.1016/j.scr.2018.101369.

Murad, J. P., Kozlowska, A. K., Lee, H. J., Ramamurthy, M., Chang, W., Yazaki, P., Colcher, D., Shively, J., Cristea, M., Forman, S. J., Priceman, S. J.  Effective Targeting of TAG72+ Peritoneal Ovarian Tumors via Regional Delivery of CAR-Engineered T Cells.  Front. Immunol. 2018; 9:2268.  doi:10.3389/fimmu.2018.02268

Demmerath, E., Bohler, S., Kunze, M., Erlacher, M.  In Vitro And In Vivo Evaluation Of Possible Pro-Survival Activities Of PGE2, EGF, TPO And FLT3L On Human Hematopoiesis.  Haematologica. November 2018; haematol.2018.191569.  doi:10.3324/haematol.2018.191569

Wagh, K., Kreider, E. F., Li, Y., Barbian, H. J., Learn, G. H., Giorgi, E., Hraber, P. T., Decker, T. G., Smith, A. G., Gondim, M. V., Gillis, L., Wandzilak, J., Chuang, G., Rawi, R., Cai, F., Pellegrino, P., Williams, I., Overbaugh, J., Gao, F., Kwong, P. D., Haynes, B. F., Shaw, G. M., Borrow, P., Seaman, M. S., Hahn, B. H., Korber, B. Completeness of HIV-1 Envelope Glycan Shield at Transmission Determines Neutralization Breadth. Cell Reports. 2018; 25(4), 893-908. doi:10.1016/j.celrep.2018.09.087

Fonseka, C. Y., Rao, D. A., Teslovich, N. C., Korsunsky, I., Hannes, S. K., Slowikowski, K., Gurish, M. F., Donlin, L. T., Lederer, J. A., Weinblatt, M. E., Massarotti, E. M., Coblyn, J. S., Helfgott, S. M., Todd, D. J., Bykerk, V. P., Karlson, E. W., Ermann, J., Lee, Y. C., Brenner, M. B., Raychaudhuri, S. Mixed-effects association of single cells identifies an expanded effector CD4+ T cell subset in rheumatoid arthritis. Science Translational Medicine. 2018; 10(463), eaaq0305. doi:10.1126/scitranslmed.aaq0305

Kuo-Jen Wu, Seong-Jin Yu, Chia-Wen Chiang, Yu-Wei Lee, B Linju Yen, Pei-Chi Tseng, Chun-Sen Hsu, Li-Wei Kuo, Yun Wang.  Neuroprotective Action of Human Wharton’s Jelly-Derived Mesenchymal Stromal Cell Transplants in a Rodent Model of Stroke.  Cell Transplant. 2018 Oct 4:963689718802754.  doi:10.1177/0963689718802754

Mathew, A. J.  Biopreservation Considerations for Regenerative Medicine GMP Manufacturing; 2018.  http://ebook.liebertpub.com/biolife-solutions/biopreservation-considerations-for-regenerative-medicine-gmp-manufacturing

Parker, S. S., Moutal, A., Cai, S., Chandrasekaran, S., Roman, M. R., Koshy, A. A., Khanna, R., Zinsmaier, K. E. and Mouneimne, G. High fidelity cryopreservation and recovery of primary rodent cortical neurons. eNeuro 2018; 10.1523/ENEURO.0135-18.2018. doi:10.1523/eneuro.0135-18.2018

Sutton, M., Kaur, S., Brown, K., Skiles, M., Folz, M., Caplan, A., and Bonfield, T.  Anti-Inflammatory Therapeutic Development and Optimization of Umbilical Cord Tissue Derived Mesenchymal Stem Cells.  Journal Of Stem Cell Research & Therapy. 2018; 8(8), 1-9.  doi: 10.4172/2157-7633.1000435

Andriolo, G., Provasi, E., Lo Cicero, V., Brambilla, A., Soncin, S., Torre, T., Milano, G., Biemmi, V., Vassalli, G., Turchetto, L., Barile, L. and Radrizzani, M.  Exosomes From Human Cardiac Progenitor Cells for Therapeutic Applications: Development of a GMP-Grade Manufacturing Method.  Front. Physiol. 2018; 9:1169.  doi:10.3389/fphys.2018.01169

Jin, J., Tarrant, R. D., Bolam, E. J., Angell-Manning, P., Soegaard, M., Pattinson, D. J., Dulal, P., Silk, S. E., Marshall, J. M., Dabbs, R. A., Nugent, F. L., Barrett, J. R., Hjerrild, K. A., Poulsen, L., Jørgensen, T., Brenner, T., Baleanu, I. N., Parracho, H. M., Tahiri-Alaoui, A., Whale, G., Moyle, S., Payne, R. O., Minassian, A. M., Higgins, M. K., Detmers, F. J., Lawrie, A. M., Douglas, A. D., Smith, R., de Jongh, W. A., Berrie, E., Ashfield, R. and Draper, S. J.  Production, quality control, stability, and potency of cGMP-produced Plasmodium falciparum RH5.1 protein vaccine expressed in Drosophila S2 cells.  Npj Vaccines. 2018; 3:32, 1-13.  doi:10.1038/s41541-018-0071-7

Liaskou, E., Patel, S. R., Webb, G., Bagkou Dimakou, D., Akiror, S., Krishna, M., Mells, G., Jones, D. E., Bowman, S. J., Barone, F., Fisher, B. A. and Hirschfield, G. M.  Increased sensitivity of Treg cells from patients with PBC to low dose IL-12 drives their differentiation into IFN-γ secreting cells.  Journal Of Autoimmunity (2018).  doi:10.1016/j.jaut.2018.07.020

HemaCare.  Regulatory T Cell Modulation of Immune Suppression.  August 2018, 1-3

George, M. J., Prabhakara, K., Toledano-Furman, N. E., Wang, Y., Gill, B. S., Wade, C. E., Olson, S. D. and Cox, C. S.  Clinical Cellular Therapeutics Accelerate Clot Formation.  STEM CELLS Translational Medicine (2018).  doi:10.1002/sctm.18-0015

Hawkins, B. J., Abazari, A., Mathew, Aby. J.  Evaluating Cell Viability After Cryopreservation.  PDA Letter.  July 2018.

Barrachina, L., Romero, A., Zaragoza, P., Rodellar, C., Vázquez, F.   Practical considerations for clinical use of mesenchymal stem cells: From the laboratory to the horse.  The Veterinary Journal. 2018; 238, 49-57.  doi:10.1016/j.tvjl.2018.07.004

Donlin, L. T., Rao, D. A., Wei, K., Slowikowski, K., McGeachy, M. J., Turner, J. D., Meednu, N., Mizoguchi, F., Gutierrez-Arcelus, M., Lieb, D. J., Keegan, J., Muskat, K., Hillman, J., Rozo, C., Ricker, E., Eisenhaure, T. M., Li, S., Browne, E. P., Chicoine, A., Sutherby, D., Noma, A., Nusbaum, C., Kelly, S., Pernis, A. B., Ivashkiv, L. B., Goodman, S. M., Robinson, W. H., Utz, P. J., Lederer, J. A., Gravallese, E. M., Boyce, B. F., Hacohen, N., Pitzalis, C., Gregersen, P. K., Firestein, G. S., Raychaudhuri, S., Moreland, L. W., Holers, M., Bykerk, V. P., Filer, A., Boyle, D. L., Brenner, M. B. and Anolik, J. H.  Methods for high-dimensional analysis of cells dissociated from cryopreserved synovial tissue.  Arthritis Res Ther. 2018; 20: 139.  doi: 10.1186/s13075-018-1631-y

Hamilton, C. A., Young, R., Jayaraman, S., Sehgal, A., Paxton, E., Thomson, S., Katzer, F., Hope, J., Innes, E., Morrison, L. J. and Mabbott, N. A.  Development of in vitro enteroids derived from bovine small intestinal crypts.  Veterinary Research. 2018; 49(1).  doi:10.1186/s13567-018-0547-5

Aijaz, A., Li, M., Smith, D., Khong, D., LeBlon, C., Fenton, O. S., Olabisi, R. M., Libutti, S., Tischfield, J., Maus, M. V., Deans, R., Barcia, R. N., Anderson, D. G., Ritz, J., Preti, R., Parekkadan, B. Biomanufacturing for clinically advanced cell therapies.  Nature Biomedical Engineering 2018; 2(6), 362-376. doi:10.1038/s41551-018-0246-6

Pham, H., Tonai, R., Wu, M., Birtolo, C., Chen, M.  CD73, CD90, CD105 and Cadherin-11 RT-PCR Screening for Mesenchymal Stem Cells from Cryopreserved Human Cord Tissue.   Int. J. Stem Cells 2018; 11(1): 26-38.  doi:10.15283/ijsc17015

HemaCare. CD34+ Hematopoietic Stem and Progenitor Cells From Different Tissue Sources For Cell Therapy. May 2018: 1-4

Fernandopulle, M., Prestil, R., Grunseich, C., Wang, C., Gan, L., Ward, M. Transcription Factor-Mediated Differentiation of Human iPSCs into NeuronsCurrent Protocols In Cell Biology 2018; 79(1), e51. doi:10.1002/cpcb.51

Lacher, M. D., Bauer, G., Fury, B., Graeve, S., Fledderman, E. L., Petrie, T. D., Coleal-Bergum, D. P., Hackett, T., Perotti, N. H., Kong, Y. Y., Kwok, W. W., Wagner, J. P., Wiseman, C. L., Williams, W. V. SV-BR-1-GM, A Clinically Effective GM-CSF-Secreting Breast Cancer Cell Line, Expresses An Immune Signature And Directly Activates CD4+ T LymphocytesFrontiers In Immunology 2018; 9(776), 1-23. doi:10.3389/fimmu.2018.00776

Lou Juliano, George Eastwood, Todd Berard, Aby J. Mathew. The Importance of Collection, Processing and Biopreservation Best Practices in Determining CAR-T Starting Material Quality. Cell Gene Therapy Insights 2018; 4(4), 327-336. doi: 10.18609/cgti.2018.032

Hiraishi, S., Schol, J., Sakai, D., Nukaga, T., Erickson, I., Silverman, L., Foley, K., Watanabe, M. Discogenic cell transplantation directly from a cryopreserved state in an induced intervertebral disc degeneration canine model. JOR Spine. (2018); 1(2), e1013. doi:10.1002/jsp2.1013

Rajamani, U., Gross, A. R., Hjelm, B. E., Sequeira, A., Vawter, M. P., Tang, J., Gangalapudi, V., Wang, Y., Andres, A. M., Gottlieb, R. A., Sareen, D. Super-Obese Patient-Derived iPSC Hypothalamic Neurons Exhibit Obesogenic Signatures and Hormone Responses.  Cell Stem Cell 2018, Vol. 22(5), 698-712.e9. doi: 10.1016/j.stem.2018.03.009

Hung, C., Xu, X., Li, L., Ma, Y., Jin, Q., Viley, A., Allen, C., Natarajan, P., Shivakumar, R., Peshwa, M. V. and Emens, L. A. Development of Anti-Human Mesothelin-Targeted Chimeric Antigen Receptor Messenger RNA–Transfected Peripheral Blood Lymphocytes for Ovarian Cancer Therapy. Human Gene Therapy. 2018; 29(5), 614-625. doi:10.1089/hum.2017.080

Habiel, D., Espindola, M., Coelho, A., & Hogaboam, C. Modeling Idiopathic Pulmonary Fibrosis in Humanized SCID Mice. The American Journal of Pathology 2018 Apr; 188(4): 891-903. doi: 10.1016/j.ajpath.2017.12.020

Yongshun Lin, Huimin Liu, Michael Klein, John Ostrominski, So Gun Hong, Ravi Chandra Yada, Guibin Chen, Keron Navarengom, Robin Schwartzbeck, Hong San, Zu-Xi Yu, Chengyu Liu, Kaari Linask, Jeanette Beers, Lugui Qiu, Cynthia E. Dunbar, Manfred Boehm, Jizhong Zou. Efficient differentiation of cardiomyocytes and generation of calcium-sensor reporter lines from nonhuman primate iPSCsScientific Reports 2018 Vol. 8(1): 1-16. doi:10.1038/s41598-018-24074-y

McCreedy, B. J., Senyukov, V. V., Nguyen, K. T.   Off the shelf T cell therapies for hematologic malignancies.  Best Practice & Research Clinical Haematology 2018; 31(2), 166-175.  doi: 10.1016/j.beha.2018.03.001.

Brown, Zhou, McIntosh, Norman, Lou, Biermann, Sullivan, Kamp, Thomson, Anagnostopoulos, Burlingham. A Humanized Mouse Model Generated Using Surplus Neonatal Tissue. Stem Cell Reports (2018). doi:10.1016/j.stemcr.2018.02.011

Rouzbeh R. Taghizadeh, Kyle J. Cetrulo, Curtis L. Cetrulo. Collagenase Impacts the Quantity and Quality of Native Mesenchymal Stem/Stromal Cells Derived during Processing of Umbilical Cord Tissue. Cell Transplantation 2018, Vol. 27(1), 181–193. doi: 10.1177/0963689717744787

Srinivasana A, Changa S-Y, Zhange S, Toh WS, Toh Y-C. Substrate stiffness modulates the multipotency of human neural crest derived ectomesenchymal stem cells via CD44 mediated PDGFR signaling. Biomaterials 2018, Vol. 167, 153-167. doi:10.1016/j.biomaterials.2018.03.022

Wagner, J., Kline, C. L., Zhou, L., Campbell, K. S., MacFarlane, A. W., Olszanski, A. J., Cai, K. Q., Hensley, H. H., Ross, E. A., Ralff, M. D., Zloza, A., Chesson, C. B., Newman, J. H., Kaufman, H., Bertino, J., Stein, M., El-Deiry, W. S.  Dose intensification of TRAIL-inducing ONC201 inhibits metastasis and promotes intratumoral NK cell recruitment.  J Clin Invest. 2018; 128(6), 2325-2338.  doi:10.1172/jci96711

Ivanics, Bergquist, Liu, Kim, Kang, Katz, Rios Perez, Thomas, Fleming, Truty. Patient-derived xenograft cryopreservation and reanimation outcomes are dependent on cryoprotectant type. Laboratory Investigation (2018). doi:10.1038/s41374-018-0042-7

Baradez, M., Biziato, D., Hassan, E., Marshall, D.  Application of Raman Spectroscopy and Univariate Modelling As a Process Analytical Technology for Cell Therapy Bioprocessing. Front. Med. 2018; 5(47), 1-14. doi:10.3389/fmed.2018.00047

Hardin, Vreeland, Clifton, Hale, Herbert, Greene, Jackson, Berry, Nichols, Yin, Yu, Wagner, Peoples. Tumor lysate particle loaded dendritic cell vaccine: preclinical testing of a novel personalized cancer vaccine. Immunotherapy (2018) 10(5), 373–382. https://doi.org/10.2217/imt-2017-0114

Mattar, M., McCarthy, C. R., Kulick, A. R., Qeriqi, B., Guzman, S., de Stanchina, E. Establishing and Maintaining an Extensive Library of Patient-Derived Xenograft Models. Frontiers in Oncology 2018, 8 (Article 19): 1-18. doi:10.3389/fonc.2018.00019

Sampath, Won, Massarelli, Li, Frankel, Vora, Maghami, Kortylewski. Combined modality radiation therapy promotes tolerogenic myeloid cell populations and STAT3-related gene expression in head and neck cancer patients. Oncotarget, 2018, Vol. 9, (No. 13), pp: 11279-11290 . https://doi.org/10.18632/oncotarget.24397

GE Healthcare Life Sciences. Workflow for primary human NK cell isolation, xeno‑free expansion, harvest, and cryopreservation.

de Smet, M., Lynch, J., Dejneka, N., Keane, M., & Khan, I.  A Subretinal Cell Delivery Method via Suprachoroidal Access in Minipigs: Safety and Surgical Outcomes.  Investigative Opthalmology & Visual Science.  January 2018; 59(1), 311.  doi:10.1167/iovs.17-22233

Griffiths, Habiel, Jaffar, Binder, Darby, Hosking, Skerra, Westall, Hogaboam, Foley. Anti-fibrotic Effects of CXCR4-Targeting i-body AD-114 in Preclinical Models of Pulmonary Fibrosis. Scientific Reports (2018) 8:3212. DOI:10.1038/s41598-018-20811-5

Henrieta Fraser, Niloufar Safinia ,Nathali Grageda, Sarah Thirkell, Katie Lowe, Laura J. Fry, Cristiano Scottá, Andrew Hope, Christopher Fisher, Rachel Hilton, David Game, Paul Harden, Andrew Bushell, Kathryn Wood, Robert I. Lechler, Giovanna Lombardi.  A Rapamycin-Based GMP-Compatible Process for the Isolation and Expansion of Regulatory T Cells for Clinical Trials.  Molecular Therapy: Methods & Clinical Development 2018 March; 3:198-209.  doi: 10.1016/j.omtm.2018.01.006

Willie Lin, Yogi Chang-Yo Hsuan, Mao-Tsun Lin, Ting-Wei Kuo, Cheng-Hsien Lin, Yu-Chin Su, Ko-Chi Niu, Ching-Ping Chang, Hung-Jung Lin. Human Umbilical Cord Mesenchymal Stem Cells Preserve Adult Newborn Neurons and Reduce Neurological Injury after Cerebral Ischemia by Reducing the Number of Hypertrophic Microglia/Macrophages.  Cell Transplantation 2017 Nov; 26(11): 1798–1810. doi: 10.1177/0963689717728936

Gouin, Peck, Antes, Johnson, Li, Vaturi, Middleton, de Couto, Walravens, Rodriguez-Borlado, Smith, Marban, Ibrahim. A comprehensive method for identification of suitable reference genes in extracellular vesicles. JOURNAL OF EXTRACELLULAR VESICLES, 2017 VOL. 6, 1347019. https://doi.org/10.1080/20013078.2017.1347019

Montanari, E., Pimenta, J., Szabó, L., Noverraz, F., Passemard, S., Meier, R. P. H., Meyer, J., Sidibe, J., Thomas, A., Schuurman, H., Gerber-Lemaire, S., Gonelle-Gispert, C. and Buhler, L. H. Beneficial Effects of Human Mesenchymal Stromal Cells on Porcine Hepatocyte Viability and Albumin Secretion. Journal of Immunology Research 2018, Article ID 1078547: 1-13. doi:10.1155/2018/1078547

Skiles, M., Brown, K., Tatz, W., Swingle, K., & Brown, H. Quantitative analysis of composite umbilical cord tissue health using a standardized explant approach and an assay of metabolic activity. Cytotherapy 2018, 20(4), 564-575. https://doi.org/10.1016/j.jcyt.2018.01.001

Petersen, Hassan, Morris, Jeffery, Lee, Jagirdar, Staton, Raikar, Spencer, Sulchek, Flowers, Waller. Improving T-cell expansion and function for adoptive T-cell therapy using ex vivo treatment with PI3Kδ inhibitors and VIP antagonists. Blood Advances 2018 2:210-223. DOI 10.1182/bloodadvances.2017011254

Zheng, Hu, Wolfe, Gonsalves, Ren, Khawli, Kaslow, Epstein. Lym-1 Chimeric Antigen Receptor T Cells Exhibit Potent Anti Tumor Effects against B-Cell Lymphoma. Int. J. Mol. Sci. 2017, 18, 2773. doi:10.3390/ijms18122773

Abazari A, Hawkins BJ, Clarke DM, Mathew AJ. Biopreservation Best Practices: A Cornerstone in the Supply Chain of Cell-based Therapies – MSC Model Case Study.  Cell Gene Therapy Insights 2017; 3(10), 853-871. DOI: 10.18609/cgti.2017.082

Lin, Hsuan, Su, Lin, Lin, Chen, Chang, Lin. CD34− human placenta-derived mesenchymal stem cells protect against heat stroke mortality in rats.  Oncotarget, 2018, Vol. 9, (No. 2), pp: 1992-2001

Weber, Romeo, Dolley-Sonneville. Human Mesenchymal Stem Cell Growth on Corning Denatured Collagen Dissolvable Microcarriers in a 5L Bioreactor. Corning Incorporated, Life Sciences, Corning, NY USA

Zeng, Tang, Toh, Wang. Generation of “Off-the-Shelf” Natural Killer Cells from Peripheral Blood Cell-Derived Induced Pluripotent Stem Cells. Stem Cell Reports Vol. 9 1–17 December 12, 2017. https://doi.org/10.1016/j.stemcr.2017.10.020

Baust, Campbell, Harbell. Best practices for cryopreserving, thawing, recovering, and assessing cells. In Vitro Cell.Dev.Biol.—Animal. DOI 10.1007/s11626-017-0201-y

Princeman, Tilakawardane, Jeang, Aguilar, Murad, Park, Chang, Ostberg, Neman, Jandial, Portnow, Forman, Brown. Regional Delivery of Chimeric Antigen Receptor– Engineered T Cells Effectively Targets HER2þ Breast Cancer Metastasis to the Brain. Clin Cancer Res. 2017 Oct 23. doi: 10.1158/1078-0432.CCR-17-2041

Giacomelli E, Bellin M, Orlova V, Mummery C. Co-Differentiation of Human Pluripotent Stem Cells-Derived Cardiomyocytes and Endothelial Cells from Cardiac Mesoderm Provides a Three-Dimensional Model of Cardiac Microtissue. Current Protocols in Human Genetics 21.9.1–21.9.22, October 2017. doi: 10.1002/cphg.46

Nham, Poznanski, Fan, Vahedi, Shenouda, Lee, Chew, Hogg, Lee, Ashlar. Ex Vivo-expanded Natural Killer Cells Derived From Longterm Cryopreserved Cord Blood are Cytotoxic Against Primary Breast Cancer Cells. Journal of Immunotherapy: Post Author Corrections: November 17, 2017. doi: 10.1097/CJI.0000000000000192

Kastrup, Haack-Sorensen, Juhl, Sondergaard, Follin, Lund, Johansen, Qayyum, Mathiasen, Jorgenson, Helqvist, Elberg, Bruunsgaard, Ekblond. Cryopreserved Off-the-Shelf Allogeneic Adipose-Derived Stromal Cells for Therapy in Patients with Ischemic Heart Disease and Heart Failure—A Safety Study. STEM CELLS TRANSLATIONAL MEDICINE 2017; 6:1963–1971. http://dx.doi.org/10.1002/sctm.17-0040

Liao, Choi, Vojnits, Xue, Aroom, Meng, Pan, Hetz, Corkins, Hughes, Triolo, Johnson, Moise, Lally, Cox, Li. Tissue Engineering to Repair Diaphragmatic Defect in a Rat Model. Stem Cells International Volume 2017, Article ID 1764523, 12 pages. https://doi.org/10.1155/2017/1764523.

Lim R, Hodge A, Moore G, Wallace E, Slevert W. A Pilot Study Evaluating the Safety of Intravenously Administered Human Amnion Epithelial Cells for the Treatment of Hepatic Fibrosis. Front. Pharmacol. 8:549.doi: 10.3389/fphar.2017.00549

Roberts, Haupt, Tucker, Grancharova, Arakaki, Fuqua, Nelson, Hookway, Ludmann, Mueller, Yang, Horwitz, Rafelski, Gunawardane. Systematic gene tagging using CRISPR/Cas9 in human stem cells to illuminate cell organization. Mol Biol Cell. 2017 Oct 15; 28(21): 2854-2874. doi: 10.1091/mbc.E17-03-0209

Laskin, Jiao, Bularte, Amaral, Furth, Akimova, Hancock, Levine, Reese, Beier. The Effects of Tacrolimus on T-Cell Proliferation Are Short-Lived: A Pilot Analysis of Immune Function Testing. Transplant Direct. 2017 Aug; 3(8): e199. doi: 10.1097/TXD.0000000000000715

Thompson, Schryver, Ehrhardt. Cell therapy post-production technologies: a select review of key innovations in the field. Cell Gene Therapy Insights 2017; 433-441.DOI: 10.18609/cgti.2017.049

Dustin R. Wakeman, Benjamin M. Hiller, David J. Marmion, Christopher W. McMahon, Grant T. Corbett, Kile P. Mangan, Junyi Ma, Lauren E. Little, Zhong Xie, Tamara Perez-Rosello, Jaime N. Guzman, D. James Surmeier, and Jeffrey H. Kordower. Cryopreservation Maintains Functionality of Human iPSC Dopamine Neurons and Rescues Parkinsonian Phenotypes In Vivo. Stem Cell Reports j Vol. 9 j 1–13 j July 11, 2017. http://dx.doi.org/10.1016/j.stemcr.2017.04.033

Fuller, Gonzalez-Molina, Erro, Mendonca, Chalmers, Awan, Poirier, Selden. Applications and optimization of cryopreservation technologies to cellular therapeutics. Cell & Gene Therapy Insights – ISSN: 2059-7800. DOI: 10.18609/cgti.2017.038

Hawkins B, Abazari A, Mathew A. Biopreservation Best Practices for Regenerative Medicine GMP Manufacturing & Focus on Optimized Biopreservation Media. Cell & Gene Therapy Insights – ISSN: 2059-7800. DOI: 10.18609/cgti.2017.035

Hsu, Prigent, Dollet, Ravau, Larbanoix, Simaeys, Bol, Gregoire, Goldman, Deblandre, Najimi, Sokal, Lombard. Long-term In Vivo Monitoring of Adult-Derived Human Liver Stem/Progenitor Cells by Bioluminescence Imaging, Positron Emission Tomography, and Contrast-Enhanced Computed Tomography. STEM CELLS AND DEVELOPMENT (Original Research Report). DOI: 10.1089/scd.2016.0338

Jeremy M. Crook and Tomaskovic-Crook (eds.), Stem Cell Banking: Concepts and Protocols, Methods in Molecular Biology, vol. 1590. doi: 10.1007/978-1-4939-6921-0_15, © Springer Science+Business Media LLC 2017

Jeremy M. Crook and Tenneille E. Ludwig (eds.), Stem Cell Banking: Concepts and Protocols, Methods in Molecular Biology, vol. 1590. DOI 10.1007/978-1-4939-6921-0_11, © Springer Science+Business Media LLC 2017

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Baust JM, Snyder KK, Mathew, AJ, Van Buskirk RG., Baust, John G. Steps to Improving Cryopreservation Outcome: Understanding key factors influencing efficacy.Bioscience Technology (2006)

Corning. Enhanced Survival of LNCaP Cells Following Cryopreservation Using the CryoStor™ CS5 Preservation Solution and Corning® CellBIND® Surface (2006)

Stylianou J, Vowels M, Hadfield K. Novel Cryoprotectant Significantly Improves the Post-thaw Recovery and Quality of HSC from CB. Cytotherapy, 8(Suppl 1):57. (2006).

Robilotto AT, Baust JM, Van Buskirk R, Baust JG. Involvement of the cysteine protease calpain family in the cell death following cryopreservation. Cell Preservation Technology 4(1) 2006.

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Van Buskirk RG, Snyder KK, Baust JG, Mathew AJ, Baust JM. Cryopreservation – It’s Not Just About Cell Yield. BioProcessing International,  April (2005).

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Snyder KK, Van Buskirk RG, Mathew AJ, Baust JG, Baust JM. Biological Packaging for the Global Cell and Tissue Therapy Markets. BioProcessing Journal May/June: 39-46 (2004).

Cho JJ, Joseph B, Sappal BS, Giri RK, Wang R, Ludlow JW, Furth ME, Susick R, Gupta S. Analysis of the functional integrity of cryopreserved human liver cells including xenografting in immunodeficient mice to address suitability for clinical applications. Liver Int. 24(4):361-70 (2004).

Sugimachi K., Sosef MN, Baust JM, Fowler A, Tompkins RG, Toner M. Long-Term Function of Cryopreserved Rat Hepatocytes in a Coculture System. Cell Transplantation 13: 187-195 (2004).

Baust JM. Molecular mechanisms of cellular demise associated with cryopreservation failure. Cell Preservation Technology 1(1): 17-31 (2002)

Baust JM, Van Buskirk RG, Baust JG. Gene activation of the apoptotic caspase cascade following cryogenic storage. Cell Preservation Technology 1(1): 63-80 (2002)

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Lee, Tsung (John) Dao.  A Comprehensive Review of Umbilical Cord Cryopreservation: Umbilical Cord Tissue Banking Cryopreserved Technology and Standard Operating Procedure.  Transfusion Vol. 58, 2018 Supplement. Pages 4A-5Av.

Clark, E., Stasko, K., and Paciulan, B.  A COMPARISON STUDY OF CURRENT FREEZING MEDIA VERSUS CRYOSTOR10 FOR CRYOPRESERVATION OF QC VIALS.  Cytotherapy. May 2018; 20(5), S62.  doi.org/10.1016/j.jcyt.2018.02.173

Pasha, Elmoazzen, Pineault. Development and testing of a stepwise thaw and dilute protocol for cryopreserved umbilical cord blood units.Transfusion. 2017 Jul;57(7):1744-1754. doi: 10.1111/trf.14136

Biunno I, Ntai A. Banking of Human Pluripotent Stem Cells. Biopreservation and Biobanking Volume 15, Number 3, 2017. DOI:10.1089=bio.2017.29021.abstracts

Fink J, Abazari A, Hawkins B, Albert M, O’Donnell K, Mathew A. Best Practices vs. Traditional Practices of Procedures and Technology for Preserving Viability and Functionality of T-Cells at Both -80 C and -190 C. Biopreservation and Biobanking Volume 15, Number 3, 2017. DOI:10.1089=bio.2017.29021.abstracts

Abazari A. Biopreservation considerations in the evolving field of cellular therapies. Cryobiology Volume 73, Issue 3, December 2016, Page 399. https://doi.org/10.1016/j.cryobiol.2016.09.007

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Almulhem N, Lutzko C, Leemhuis T. Analysis of HPC and HSC post-thaw viability, clonogenic capacity, and proliferative potential after cryopreservation in comercially available media. Cytotherapy: April 2013 Volume 15, Issue 4, Supplement, Page S29. DOI: http://dx.doi.org/10.1016/j.jcyt.2013.01.109

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Yasuhara T, Matsukawa N, Hara K, Maki M, Ali MM, Yu SJ, Bae E, Yu G, Xu L, McGrogan M, Bankiewicz K, Case C, Borlongan CV. Notch-Induced Rat and Human Bone Marrow Stromal Cell Grafts Reduce Ischemic Cell Loss and Ameliorate Behavioral Deficits in Chronic Stroke Animals. Stem Cells and Development. December 2009, 18(10): 1501-1514. doi:10.1089/scd.2009.0011.

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The Effect of Cryomedia Selection and Transient Warming Events on Post-Cryopreservation Human MSC Function

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Exploration of Shipping Conditions to Retain Viable Recovery for Umbilical Tissue

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Transition to GMP, chemically-defined, xeno-free cryopreservation media increases post-thaw functionality of clinically relevant cell banks

Cryopreservation and Transport of Jurkat T Cells Using Current and Optimized Practices: The Impact of Temperature and Length of Storage




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Continued Outgrowth of Cord Tissue-Derived Cells Over Multiple Platings From Cord Tissue Cryopreserved as a Composite Material


Scalable Xeno-Free Expansion of Human Mesenchymal Stromal Cells in a Single-Use, Vertical-Wheel Bioreactor System

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AABBPoster101815.Final Identification of CD73, CD90 and CD105 by qPCR in Cryopreserved Human Cord Tissue

Scalability of Allogeneic Stem Cell Manufacturing

Development of a quantitative measurement of mesenchymal stem cells explant outgrowth from umbilical cord tissue

ISCT Poster FvT 052115 FINAL_roosterbio_icon Cryopreserved hMSCsmaintain comparable in vitro functional activity compared to fresh hMSCs

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2014 BEST 65 POSTER Cryopreservation of donor lymphocyte clinical products with conventional 10% DMSO and CryoStor®

Optimisation of cGMP Cryopreservation of hESC using CryoStor® Part 1

Optimisation of cGMP Cryopreservation of hESC-Roslin Cells Optimisation of cGMP Cryopreservation of hESC using CryoStor® Part 2

Poster_Eyrich_ISCT2013_135 GMP-Compliant Production Dendritic Cells

2013CBForum poster abicon Reduced Engraftment Times

IBET_cryopreservation of cardiomyocytes ECI_Page_1 Cryopreservation Of Cardiomyocytes

Effect of Cryopreservation on Cell Viability, Recovery, and Proliferation

Stem Cell Cryopreservation Stability

Gel Improves Stability and Recovery

Cord-Blood-Stem-Cell-Banking_thumb Cord Blood Stem Cell Banking Using CryoStor

Maximizing-Stability-of-Human-Stem-Cells.png Cryopreservation of Adherent Cells Using CryoStor

Cryopreservation Strategies of Adherent Cells

Improving Cryopreservation Yield

Safety Study of Intravenous Injection of Solutions

Accelerated Stability Study

Mitochondrial-Associated Pathway of Apoptosis Contributes to Failure

Cellular Proteomic Alterations Influence Cell Survival

Calpain Activation Contributes to Cell Death

Evolution of Biological Packaging and Biopreservation

Cryopreservation of Hepatocytes

Improved Stability Validation of Peripheral Blood Cell Products

Standardized Drug-Testing Using Tumor Samples

Considerations for cGMP Production of Patient-Specific Cell Therapeutics – A PACT Case Study. Derek JH. Webinar(slides). (2014).

Human-Derived Raw Materials: Controlled, Consistent Collections Enable Successful Manufacturing of Cell-Based Regenerative Medicine Products.

New cryogenic container for clinical cryopreservation of biomaterials


Managing of a Stem Cell Biorepository

Hypothermic Storage & Shipping Media

Stroncek, D., Mathew, A., & McKenna, D. Cell therapies for trauma and critical care medicine: critical issues in translation for cellular and novel therapies in trauma and critical care. Transfusion. 2019; 59(S1), 854-857. doi:10.1111/trf.14832

Mathew, A. J.  Biopreservation Considerations for Regenerative Medicine GMP Manufacturing; 2018.  http://ebook.liebertpub.com/biolife-solutions/biopreservation-considerations-for-regenerative-medicine-gmp-manufacturing

Harrison, R., Lugo Leija, H. A., Strohbuecker, S., Crutchley, J., Marsh, S., Denning, C., El Haj, A., Sottile, V.  Development and validation of broad-spectrum magnetic particle labelling processes for cell therapy manufacturing.   Stem Cell Res Ther, 2018 Sept 26; 9(1):248.  doi:10.1186/s13287-018-0968-0.

Peltzer, J., Aletti, M., Frescaline, N., Busson, E., Lataillade, J. and Martinaud, C. Mesenchymal Stromal Cells Based Therapy in Systemic Sclerosis: Rational and Challenges. Front. Immunol. September 2018; 9:2013. doi:10.3389/fimmu.2018.02013

Barrachina, L., Romero, A., Zaragoza, P., Rodellar, C., Vázquez, F.  Practical considerations for clinical use of mesenchymal stem cells: From the laboratory to the horse.  The Veterinary Journal. 2018; 238, 49-57.  doi:10.1016/j.tvjl.2018.07.004

Lou Juliano, George Eastwood, Todd Berard, Aby J. Mathew. The Importance of Collection, Processing and Biopreservation Best Practices in Determining CAR-T Starting Material Quality. Cell Gene Therapy Insights 2018; 4(4), 327-336. doi: 10.18609/cgti.2018.032

Hiraishi, S., Schol, J., Sakai, D., Nukaga, T., Erickson, I., Silverman, L., Foley, K., Watanabe, M. Discogenic cell transplantation directly from a cryopreserved state in an induced intervertebral disc degeneration canine model. JOR Spine. (2018); 1(2), e1013. doi:10.1002/jsp2.1013

Mattar, M., McCarthy, C. R., Kulick, A. R., Qeriqi, B., Guzman, S., de Stanchina, E. Establishing and Maintaining an Extensive Library of Patient-Derived Xenograft Models Frontiers in Oncology 2018, 8 (Article 19): 1-18. doi:10.3389/fonc.2018.00019

Wang, Penland, Gokce, Croote, Quake. High fidelity hypothermic preservation of primary tissues in organ transplant preservative for single cell transcriptome analysis. BMC Genomics (2018) 19:140. https://doi.org/10.1186/s12864-018-4512-5

Abazari A, Hawkins BJ, Clarke DM, Mathew AJ. Biopreservation Best Practices: A Cornerstone in the Supply Chain of Cell-based Therapies – MSC Model Case Study.  Cell Gene Therapy Insights 2017; 3(10), 853-871. DOI: 10.18609/cgti.2017.082

Kozuka, He, Koo-McCoy, Kumaraswamy, Nie, Shaw, Chan, Leadbetter, Lewis, Zhong, Charmot, Balaa, King, Caldwell, Siegel. Development and Characterization of a Human and Mouse Intestinal Epithelial Cell Monolayer Platform. Stem Cell Reports Vol. 9 1–15 December 12, 2017. https://doi.org/10.1016/j.stemcr.2017.10.013

Gniadek, Garritsen, Stroncek, Szczepiorkowski, McKenna. Optimal Storage Conditions for Apheresis Research (OSCAR): a Biomedical Excellence for Safer Transfusion (BEST) Collaborative study. Transfusion. 2017 Dec 6. doi: 10.1111/trf.14429

Ayoub, Lee, Driesbaugh, Anselmo, Hughes, Ferrari, Gorman, Sacks. Regulation of valve interstitial cell homeostasis by mechanical deformation: implications for heart valve disease and surgical repair. J. R. Soc. Interface 14: 20170580 http://dx.doi.org/10.1098/rsif.2017.0580

Giulia Leoni, Alex Lyness, Patrick Ginty, Rindi Schutte, Gopalan Pillai, Gayatri Sharma, Paul Kemp, Natalie Mount, Michaela Sharpe.  Preclinical development of an automated injection device for intradermal delivery of a cell-based therapy.  Drug Delivery and Translational Research. (2017) 7:695–708.  doi: 10.1007/s13346-017-0418-z

Raffia, Nienow, Hewitt, Cookman. The role of biopreservation in cell and gene therapy bioprocessing. Cell & Gene Therapy Insights – ISSN: 2059-7800. DOI: 10.18609/cgti.2017.037

Hawkins B, Abazari A, Mathew A. Biopreservation Best Practices for Regenerative Medicine GMP Manufacturing & Focus on Optimized Biopreservation Media. Cell & Gene Therapy Insights – ISSN: 2059-7800. DOI: 10.18609/cgti.2017.035

Sinden J, Hicks C, Stroemer P, Vishnubhatla I, Corteling R. Human Neural Stem Cell Therapy for Chronic Ischemic Stroke: Charting Progress From Laboratory to Patients. Stem Cells Dev. 2017 Jul 1;26(13):933-947. doi: 10.1089/scd.2017.0009

Der, Ranabothu, Suryawanshi, Akat, Clancy, Morozov, Kustagi, Czuppa, Izmirly, Belmont, Wang, Jordan, Bornkamp, Nwaukoni, Martinez, Goilav, Buyon, Tuschl, Putterman. Single cell RNA sequencing to dissect the molecular heterogeneity in lupus nephritis. JCI Insight. 2017;2(9):e93009. doi: https://doi.org/10.1172/jci.insight.93009

Kayiran O. Evolution of Hair Transplantation. Clinics in Surgery Article; Department Plastic and Reconstructive Surgery Clinic, Ankara Research and Training Hospital, Turkey

Whitehouse M, Howells N, Parry M, Austin E, et al. Repair of Torn Avascular Meniscal Cartilage Using Undifferentiated Autologous Mesenchymal Stem Cells: From In Vitro Optimization to a First-in-Human Study. STEM CELLS TRANSLATIONALMEDICINE 2016; 00:00–00. DOI: 10.1002/sctm.16-0199

Fernandes MC, Dillon LA, Belew AT, Bravo HC, Mosser DM, El-Sayed NM.   Dual transcriptome profiling of Leishmania-infected human macrophages reveals distinct reprogramming signatures.  mBio (2016) 7(3):e00027-16.  doi:10.1128/mBio.00027-16.

Williams LB, Co C, Koenig JB, Tse C, Lindsay E, Koch TG.  Response to Intravenous Allogeneic Equine Cord Blood-Derived Mesenchymal Stromal Cells Administered from Chilled or Frozen State in Serum and Protein-Free Media. Frontiers in Veterinary Science. 2016; 3: 56. Published online 22 Jul 2016; doi: 10.3389/fvets.2016.00056

Baijnath S, Shobo A, Bester LA, et al.  Small molecule distribution in rat lung: a comparison of various cryoprotectants as inflation media and their applicability to MSI. Journal of Molecular Histology (2016) 47: 213.  doi:10.1007/s10735-016-9658-3.

Correia C, Koshkin A, Carido M, Espinha N, Saric T, Lima P, Serra M, Alves P Effective Hypothermic Storage of Human Pluripotent Stem Cell-Derived Cardiomyocytes Compatible With Global Distribution of Cells for Clinical Applications and Toxicology Testing STEM CELLS TRANSLATIONAL MEDICINE 2016; 5:658–669 DOI: 10.5966/sctm.2015-0238

Henry TD, Schaer GL, DeMaria A, Recker D, Remmers AE, Goodrich J, Patel A.  The iXCELL-DCM Trial: Rationale and Design.  Cell Transplant. 2016 Mar 22. [Epub ahead of print].  DOI:10.3727/096368916X691295.

Blázquez R, Sánchez-Margallo FM, Crisóstomo V, et al. Intrapericardial Delivery of Cardiosphere-Derived Cells: An Immunological Study in a Clinically Relevant Large Animal Model. PLoS ONE. 2016;11(2):e0149001.Published online 11 Feb 2016.  doi:10.1371/journal.pone.0149001.

Rose PT.  Hair restoration surgery: challenges and solutions. Clinical, Cosmetic and Investigational Dermatology. 2015; 8: 361-370. Published online 2015 Jul 15. doi:  10.2147/CCID.S53980

Blázquez R, Sánchez-Margallo FM, Crisóstomo V, Báez C, Maestre J, García-Lindo M, et al. (2015) Intrapericardial Administration of Mesenchymal Stem Cells in a Large Animal Model: A Bio-Distribution Analysis. PLoS ONE 10(3): e0122377. DOI:10.1371/journal.pone.0122377

Anderson MJ, David ML, Scholz M, Bull SJ, Morse D, Stevens MH, Peterson ML. Efficacy of Skin and Nasal Povidone-Iodine Preparation against mupirocin resistant MRSA and Staphylococcus aureus within the anterior nares. AAC Accepted Manuscript Posted Online 2 March 2015 Antimicrob. Agents Chemother. DOI:10.1128/AAC.04624-14

AlMulhem, N.A. (2015). Cryopreservation and Hypothermal Storage of Hematopoietic Stem Cells (Master Thesis). Retrieved from https://etd.ohiolink.edu/!etd.send_file?accession=ucin1439307244&disposition=attachment

AGE Day, Bhangra KS, Murray-Dunning C, Thanabalasundaram L, Grace N, Cameron G, Stevanato L, Sinden J, Phillips JB. Hypothermic and cryogenic preservation of artificial neural tissue made using differentiated CTX human neural stem cells in collagen gels. European Cells and Materials. Vol 29. Suppl. 3, (2015). Pg 44.

Antunes, Santos. Developing an Advanced Therapy Medicinal Product (ATMP) for the treatment of GvHD. Dissertações de Mestrado.

Cooley, J. Bio-Enhanced Hair Restoration. Hair Transplant Forum International Volume 24, Number 4

Carver GE, Locknar SA, Morrison WA, Ramanujan VK, Farkas DL. High-speed multispectral confocal biomedical imaging. Journal of Biomedical Optics 19(3), 036016 (March 2014). DOI: 10.1117/1.JBO.19.3.036016

Bartunek J, Behfar A, Dolatabadi D, Vanderheyden M, Ostojic M, Dens J, et al. Cardiopoietic Stem Cell Therapy in Heart Failure. Journal of the American College of Cardiology Vol. 61, No. 23, 2013, 0735-1097.

Petrof G, Martinez-Queipo M, Mellerio J, Kemp P, McGrath J Fibroblast cell therapy enhances initial healing in recessive dystrophic epidermolysis bullosa wounds: results of a randomized, vehicle-controlled trial. British Journal of Dermatology (2013) 169, pp1025–1033. DOI 10.1111/bjd.12599

Usta OB, Kim Y, Ozer S, Bruinsma BG, Lee J, Demir E, et al. (2013) Supercooling as a Viable Non-Freezing Cell Preservation Method of Rat Hepatocytes. PLoS ONE 8(7): e69334. doi:10.1371/journal.pone.0069334

Campbell SD, Crafford A, Williamson BL, Kharasch ED. Mechanism of Autoinduction of Methadone N-Demethylation in Human Hepatocytes. Published online before print June 3, 2013, doi: 10.1213/​ANE.0b013e3182918252. A & A July 2013 vol. 117 no. 1 52-60

Randelli P, Conforti E, Piccoli M, Ragone V, Creo P, Cirillo F, Masuzzo P, Tringali C, Cabitza P, Tettamanti G, Gagliano N, Anastasia L. Isolation and Characterization of 2 New Human Rotator Cuff and Long Head of Biceps Tendon Cells Possessing Stem Cell–Like Self-Renewal and Multipotential Differentiation Capacity. The American Journal of Sports Medicine. 2013. DOI: 10.1177/0363546512473572 2013.

Mathew AJ. A review of cellular biopreservation considerations during hair transplantation. Hair Transplant Forum International, 2013; 23(1):1-11.

Zhuang, Pound, Willems, Taylor, Forrester, Gregory. Pure populations of murine macrophages from cultured embryonic stem cells. Application to studies of chemotaxis and apoptotic cell clearance. J Immunological Methods: 2012 Nov 30;385(1-2):1-14. doi: 10.1016/j.jim.2012.06.008

Smith EJ, Stroemer RP, Gorenkova N, Nakajima M, Crum WR, Tang E, Stevanato L, Sinden JD, Modo M. Implantation Site and Lesion Topology Determine Efficacy of a Human Neural Stem Cell Line in a Rat Model of Chronic Stroke. Published online before print: 22 MAR 2012 DOI: 10.1002/stem.1024. Stem Cells Volume 30, Issue 4, pages 785–796, April 2012.

Spitzer TL, Rojas A, Zelenko Z, Aghajanova L, Erikson DW, Barragan F, Meyer M, Tamaresis JS, Hamilton AE, Irwin JC, Giudice LC. Perivascular Human Endometrial Mesenchymal Stem Cells Express Pathways Relevant to Self-Renewal, Lineage Specification, and Functional Phenotype. Biology of Reproduction. 2012 Feb 29; 86(2):58. Published 9 November 2011. Print 2012 Feb.; DOI:10.1095/biolreprod.111.095885.

Ginis I, Grinblat B, Shirvan MH. Evaluation of Bone Marrow-Derived MSC after Cryopreservation and Hypothermic Storage in Clinically Safe Medium. Tissue Engineering Part C: Methods. (2012) Volume 18, Number 6, 2012. DOI: 10.1089/ten.2011.0395.

Akabawy GE, Rattray I, Johansson SM, Gale R, Bates G, Modo M. Implantation of undifferentiated and predifferentiated human neural stem cells in the R6/2 transgenic mouse model of Huntington’s disease. BMC Neuroscience (2012), 13:97; DOI:10.1186/1471-2202-13-97.

Povsic TJ, O’Connor CM, Henry T, Taussig A, et al. A double-blind, randomized, controlled, multicenter study to assess the safety and cardiovascular effects of skeletal myoblast implantation by catheter delivery in patients with chronic heart failure after myocardial infarction. American Heart Journal, October 2011. Volume 162, Number 4, 654-662. DOI: http://dx.doi.org/10.1016/j.ahj.2011.07.020.

Powell RJ, Comerota AJ, Berceli SA, Guzman R, Henry TD, Tzeng E, Velazquez O, Marston WA, Bartel RL, Longcore A, Stern T, Watling S. Interim analysis results from the RESTORE-CLI, a randomized, double-blind multicenter phase II trial comparing expanded autologous bone marrow-derived tissue repair cells and placebo in patients with critical limb ischemia. Journal of Vascular Surgery. (2011) Oct;54(4):1032-41.

Woods EJ, Thirumala S. Packaging Considerations for Biopreservation. Transfusion Medicine and Hemotherapy. 2011; 38(2): 149–156. DOI: 10.1159/000326083

Lowe NJ, Lowe PL, St. Clair Roberts J. Phase IIa Open-Label Dose-Escalation Pilot Study Using Allogeneic Human Dermal Fibroblasts for Nasolabial Folds. Dermatologic Surgery. (2010). Volume 36, Issue 10, October 2010, Pages: 1578–1585. Epub 2010 Aug 16. DOI:10.1111/j.1524-4725.2010.01686.x.

Nève N, Kohles SS, Winn SR, Tretheway DC. Manipulation of Suspended Single Cells by Microfluidics and Optical Tweezers. (2010). Cellular and Molecular Bioengineering. Vol 3, No 3, 213-228. DOI:10.1007/s12195-010-0113-3.

Mathew, AJ.  I’m Losing Cell Viability and Function at Different Points in My Process, and I Don’t Know Why! BioProcess International, 8 (6): 54-7 (2010).

Yang Y, Steeg J, Honaramooz A. The effects of tissue sample size and media on short-term hypothermic preservation of porcine testis tissue. (2010). Cell and Tissue Research. 2010 May;340(2):397-406. Epub 2010 Mar 16.

Parsley WM, Perez-Meza D. Review of Factors Affecting the Growth and Survival of Follicular Grafts. (2010). J Cutan Aesthet Surg. 2010 May–Aug; 3(2): 69–75. DOI:10.4103/0974-2077.69014.

Stéphenne X, Najimi M, Sokal EM. . Hepatocyte Cryopreservation: Is it Time to Change the Strategy? (2010). World Journal of Gastroenterology. 2010 January 7; 16(1): 1-14.

Ostrowska A, Gu K, Bode DC, Van Buskirk RG. Hypothermic storage of isolated human hepatocytes: a comparison between University of Wisconsin solution and a hypothermosol platform. Archives of Toxicology. 2009 May;83(5):493-502.

Perry BC, Zhou D, Wu X, Yang FC, Byers MA, Chu TMG, Hockema JJ, Woods EJ, Goebel WS. Collection, Cryopreservation, and Characterization of Human Dental Pulp–Derived Mesenchymal Stem Cells for Banking and Clinical Use. Tissue Engineering Part C: Methods. June 10, 2008, 14(2): 149-156. DOI:10.1089/ten.tec.2008.0031.

Mathew, AJ, Snyder, KK, Baust, JM. Methods for the Effective Preservation of Cell and Tissue Samples. BioTechniques Dec 2006; p.27

Van Buskirk RG, Snyder KK, Mathew AJ, Baust JG, Baust JM. Navigating the Postpreservation Viability Fog. (2006). GEN. Nov 1, 2006 (Vol. 26, No. 19)

Baust, JM. Advances in Media for Cryopreservation and Hypothermic Storage. BioProcess International Supplement, June: 46-56 (2005).

Baust JM, Snyder KK, Mathew, AJ, Van Buskirk RG., Baust, John G. Steps to Improving Cryopreservation Outcome: Understanding key factors influencing efficacy. Bioscience Technology (2006)

Snyder KK, Van Buskirk RG, Baust JM, Mathew AJ, Baust JG. Enhanced Hypothermic Storage of Neonatal Cardiomyocytes. Cell Preservation Technology 3(1):61-74 (2005).

Cooley, J. Ischemia-Reperfusion Injury and Graft Storage Solutions. Hair Transplant Forum International Volume 14, Number 4

Snyder, K.K., Van Buskirk, R.G., Baust, J.M., Mathew, A.J., Baust, J.G. Biological Packaging for the Global Cell and Tissue Therapy Markets. BioProcessing Journal May/June: 39-46 (2004).

Baust JM, Mathew AJ, Snyder K, Liu E, Van Buskirk RG, Hardy M, Baust JG. Transplantation Diagnostics: A Preliminary Analysis Using Protein Microarray to Determine Kidney Status Prior To and Following Implantation. Cell Preservation Technology 2(2): 81-90 (2004).

Bessems M, Doorschodt BM, van Vliet AK, van Gulik TM. Preservation of rat livers by cold storage: a comparison between the University of Wisconsin solution and HypoThermosol. Ann Transplant 9(2): 35-7 (2004).

Mathew AJ, Baust JM, Van Buskirk RG, Baust JG. Cell Preservation in Reparative and Regenerative Medicine: Evolution of Individualized Solution Composition. Tissue Engineering 10(11/12): 1662-1671 (2004).

Van Buskirk RG, Baust JM, Snyder KK, Mathew AJ, Baust JG. . Hypothermic Storage and Cryopreservation – The Issues of Successful Short-term and Long-term preservation of cells and tissues. Bioprocessing International 2(10): 42-49 (2004).

Mathew AJ, Van Buskirk RG, Baust JG. Improved Hypothermic Preservation of Human Renal Cells Through Suppression of Both Apoptosis and Necrosis. Cell Preservation Technology 1(4):239-251 (2003).

Ikonomovic M, Kelly KM, Hentosz TM, Shih SR, Armstrong DM, Taylor MJ. Ultraprofound cerebral hypothermia and blood substitution with an acellular synthetic solution maintains neuronal viability in rat hippocampus. CryoLetters 22: 19-26 (2001).

Dahdah NS, Taylor MJ, Russo P, Wagerle LC. . Effects of HypoThermosol, an experimental acellular solution for tissue preservation and cardiopulmonary bypass, on isolated newborn lamb coronary vessels subjected to ultra profound hypothermia and anoxia. Cryobiology, 39, 58-68 (1999).

Mathew AJ, Hollister WR, Addona T, Baust JG, Van Buskirk RG. Vitamin E and EDTA improve the efficacy of HypoThermosol – implication of apoptosis. In Vitro and Molecular Toxicology 12(3): 163-72 (1999).

Mathew AJ, Baust JG, Van Buskirk RG. Optimization of hypothermosol for the hypothermic storage of cardiac cells – addition of EDTA. In Vitro Toxicology. 10(4): 407-415, Winter. (1997).

Van Buskirk RG, Rauch J, Robert S, Taylor MJ, Baust JG. Assessment of hypothermic storage of normal human epidermal keratinocytes (NHEK) using alamar blue. In Vitro Toxicology, 9(3), 297-303 (1996).

Simon D, Taylor MJ, Elrifai AM, et al. Hypothermic blood substitution enables resuscitation after hemorrhagic shock and 2 hours of cardiac arrest. ASAIO J. 1995 Jul-Sep;41(3):M297-300.

Taylor MJ, Bailes JE, Elrifai AM, Shih SR, Teeple E, Leavitt ML, Baust JG, Maroon JC. A New Solution for Life Without Blood: Asanguineous Low-Flow Perfusion of a Whole-Body Perfusate During 3 Hours of Cardiac Arrest and Profound Hypothermia. Circulation 91(2): 431-44 (1995).

Cook JR, Eichelberger H, Robert S, Rauch J, Baust JG, Taylor MJ, Van Buskirk RG. Cold-storage of synthetic human epidermis in HypoThermosol. Tissue Engineering 1(4): 361-77 (1995).

Rhoads LS. Use of Hypothermically Stored Keratinocyte Cultures for In Vitro Toxicology. Journal of Toxicology: Cutaneous and Ocular Toxicology. 13(3): 231-48 (1994).

Abazari A. Biopreservation considerations in the evolving field of cellular therapies. Cryobiology Volume 73, Issue 3, December 2016, Page 399. https://doi.org/10.1016/j.cryobiol.2016.09.007

Liotta A, Sandersen C, Ceusters J, Girod M, Peeters D, Bolen G. Computed tomography-guided injection of muscle-derived mesenchimal stem cells in the intervertebral disc of dogs affected by natural disc degeneration: clinical safety and intervertebral disc imaging assessment.

Rossetti T, Nichols F, Modo M.  Intracerebral Cell Implantation: Preparation and Characterization of Cell Suspensions.  Cell Transplant. 2016;25(4):645-664.  doi: 10.3727/096368915X690350

Herias M. Investigation of HypoThermosol® as a delivery medium for cardiomyocyte progenitor cellsCytotherapy 16(4):S38 (2014). DOI: http://dx.doi.org/10.1016/j.jcyt.2014.01.130

Honaramooz, Yang. Isolation of Porcine Testis Cells from Fresh and Preserved Tissues. Biology of Reproduction, Volume 81, Issue Suppl_1, 1 July 2009, Page 688. https://doi.org/10.1093/biolreprod/81.s1.688

Snyder KK, Baust JM, Van Buskirk RG, Baust JG. Improved Hypothermic Storage of Sensitive Primary Cell Systems. Cytotherapy, Vol. 7 Supp. 1, pg. 2, 2005.

Snyder KK, Baust JM, Van Buskirk RG, Baust JG. Assessment of Function and Metabolic Activity of Neonatal Cardiomyocytes Following Hypothermic Storage. Cryobiology, Vol. 47 No. 3, pg. 273, 2003. (P.L. Steponkus Crystal Award Recipient)

Mathew AJ, Baust JG, Van Buskirk RG. Enhanced hypothermic preservation of multiple human cell types. Cryobiology 43:383 (2001).

Mathew AJ, Baust JG, Van Buskirk RG. Enhanced hypothermic preservation of human renal cells. Cryobiology 43:357 (2001).

Mathew, AJ, Baust JM, Hollister WR, Al-Abdullah IH, Ricordi C, Van Buskirk RG, Baust JG. Molecular biological analysis of cell death leads to improvement in the hypothermic solution, HypoThermosol. Cryobiology 41:335-6 (2000).

Mathew AJ, Hollister WR, Baust JG, Van Buskirk RG. Continued optimization of the cold storage solution HypoThermosol and implications of apoptosis. Cryobiology 37:398-9 (1998).

Wagerle LC, Taylor MJ, Dahdah NS, Russo P.   Effects of ultraprofound hypothermia on the ex vivo contractile response of cerebral arteries: Relevance to cerebrovascular dysfunction during cardiopulmonary bypass. Cryobiology 35:346 (1997).

Taylor MJ, Snyder M, Machen L, Clark RE, Baust JG. Functional survival of isolated rabbit hearts after cold storage in HypoThermosol modified with compounds that might promote retention and repletion of high energy phosphates. Cryobiology 33:620-1, (1996).

Elrifai AM, Taylor MJ, Shih SR, Bailes JE, Kiya N, Wilberger JE, Maroon JC.  Hypothermic preservation of immature spinal cord using HypoThermosol for transplantation in the rat. Cryobiology 33:675 (1996).

Van Buskirk RG, Taylor MJ, Shen R, Tang W, Mathew AJ, Baust JG. Optimization of HypoThermosol using cultured cardiomyocytes and the fluorescent multiple endpoint assay. Cryobiology 32:590 (1995).

Van Buskirk RG, Taylor MJ, Rauch J, Roberts S, Baust JG. Preservation of synthetic human skin in HypoThermosol. Cryobiology 32:591 (1995).

Taylor MJ, Clark RE, Snyder M, Machen L, Baust JG. Static cold storage of isolated rabbit hearts in HypoThermosol: Influence of butanedione monoxime during short-term storage. Cryobiology 32:538-9 (1995).

Taylor MJ, Clark RE, Baust JG. The importance of adenosine and glutathione as components of a new hypothermic blood substitute (HypoThermosol): Evaluation of myocardial protection at 7°C using an isolated working rabbit heart model. Cryobiology 30:593-4 (1994).

Taylor MJ, Simon D, Elrifai AM, Shih SR, Bailes JE, Baust JG, Maroon JC, Diamond DL. A feasibility study in a canine model for using profound hypothermia and blood substitution with HypoThermosol to enable resuscitation after hemorrhagic shock. Cryobiology 31:592-3 (1994).