Mesenchymal Stem Cells have been the cell type of interest for the following articles recently entered into BioLife’s growing Evidence library.
1. Sutton, M. T., Kaur, S., Brown, K. S., Skiles, M. L., Folz, M. A., Caplan, A. I. and Bonfield, T. L.. (2018). Anti-Inflammatory Therapeutic Development and Optimization of Umbilical Cord Tissue Derived Mesenchymal Stem Cells. Journal Of Stem Cell Research & Therapy, 08(08). doi:10.4172/2157-7633.1000435.
Sutton et. al. investigated human mesenchymal stem cells (hMSCs) derived from human umbilical cord tissue (HCT) for cytokine production in response to bacterial analogs lipopolysaccharide and peptidoglycan, for production of anti-inflammatory cytokines IL-6 and IL-8 by growth optimized hMSCs, and for functional capacity by hMSCs’ ability to influence IL-6 and IL-8 cytokine production in A549 epithelial cells. hMSCs used for the investigation were isolated by explant outgrowth from donated human umbilical cord tissue cryopreserved in CryoStor® CS10.
2. Lee, Tsung (John) Dao. (2018). A Comprehensive Review of Umbilical Cord Cryopreservation: Umbilical Cord Tissue Banking Cryopreserved Technology and Standard Operating Procedure. Transfusion Vol. 58, 2018 Supplement. Pages 4A-5Av. doi:10.1111/trf.14898.
Lee presents cryopreservation of fresh umbilical cord tissue (UC) as an alternative to the time and labor intensive direct isolation of mesenchymal stem cells (MSC). Lee’s method is applicable to large scale production of MSCs. Lee’s method includes mincing UC, addition of CryoStor® CS10, pre-incubation for sixty minutes at four degrees centigrade followed by cryopreservation using a controlled rate freezer. Pre-incubated and cryopreserved in CS10, UC maintains vitality, viability and integrity ensuring MSC isolation and culture.
3. Kuo-Jen Wu, Seong-Jin Yu, Chia-Wen Chiang, Yu-Wei Lee, B Linju Yen, Pei-Chi Tseng, Chun-Sen Hsu, Li-Wei Kuo, Yun Wang. (2018). Neuroprotective Action of Human Wharton’s Jelly-Derived Mesenchymal Stromal Cell Transplants in a Rodent Model of Stroke. Cell Transplant. 2018 Oct 4:963689718802754. doi.org/10.1177/0963689718802754
Wu et. al. demonstrated in a rat model of stroke that intracerebral implantation of human Wharton’s jelly-derived mesenchymal stem cells (hWJ-MSCs) conveys neuroprotective effects. Human WJ-MSCs were recovered from umbilical cords processed to remove arteries and veins, minced, enzymatically digested, and placed in cell culture to facilitate outgrowth and expansion of MSCs. Primary MSCs were harvested for cryopreservation in CryoStor® CS10.
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