BioPreservation Blog 1/30/2019

Disease models facilitate understanding the pathogenesis of disease, identification and development of potential interventions and evaluation of new treatments. BioLife Solutions maintains an online, searchable, Evidence Library that contains over 365 articles, abstracts and posters citing Biopreservation efficacy of CryoStor® and HypoThermosol®. Recently added to the Evidence Library, the journal article authored by Xinyue Bai et al. describes the derivation of an induced pluripotent stem cell line that provides a new tool to explore the pathogenesis of pathological myopia. Bai, X., Yang, X., Cheng, Y., Chen, L. Establishment of an induced pluripotent stem cell line (FDEENTi001-A) from a patient with pathological myopia. Stem Cell Research. 2019; 34, 101369. doi:10.1016/j.scr.2018.101369 Pathological myopia (PM) is extreme nearsightedness characterized by elongation of the eyeball, and retinal abnormalities including thinning, neovascularization and detachment. Complications from PM are a leading cause of loss of best-corrected visual acuity (BCVA)1. Genetic and environmental factors are thought to be involved in disease etiology2. PM may be induced in animal models (chickens, tree shrews, guinea pigs, marmosets, monkeys, kestrels, rabbits and fish) using corrective lenses, surgery, drugs and genetic engineering (in mice)3. Induced pluripotent stem cells (iPSCs) from an individual with PM may provide an additional resource for exploration of pathogenesis. Bai et al. established iPSC line (FDEENTi001-A) by reprogramming peripheral blood mononuclear cells (PBMCs) from a human patient with PM. PBMCs were isolated and cultured for transduction with Sendai viral vectors expressing OCT4, SOX2, KLF4 and C-MYC. Twenty-seven days post transduction, ESC-like colonies were picked. Established iPSCs were passaged every 4 – 6 days prior to being frozen in CryoStor CS10. References 1. Kyoko Ohno-Matsui. Pathologic myopia. Ann Eye Sci. 2018; 3(8), 1-11. doi: 10.21037/aes.2018.01.03 2. Guilherme Macedo Souza, Cecília Oliveira Santos, Ricardo Luz Leitão Guerra, Roberto Lorens Marback, Otacílio de Oliveira Maia Jr. Degenerative Myopia Progression: Case Report and Review. Med Clin Rev. 2015; 1(1). doi: 10.21767/2471-299X.1000002 3. Frank Schaeffel, Marita Feldkaemper. Animal models in myopia research. Clin Exp Optomy. 2015; 98(6), 507-517. doi:10.1111/cxo.12312 What disease state and cell type are you interested in? Search our Evidence Library. Help is available to answer all your Biopreservation questions. Ask the Scientists. Call 1.866.6543 (toll-free in North America) or direct: 1.425.402.1400, today!

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